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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2002-7-10
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pubmed:abstractText |
This article describes a DNA-based vaccination strategy evaluated ex vivo with human cells. The vaccine was prepared by transferring tumor-derived genomic DNA to PCI-13 cells, a highly immunogenic tumor cell line ("recipient cell"), which had been genetically modified to secrete IL-2 (PCI-13/IL-2). PCI-13 cells expressed class I MHC determinants (HLA-A2) shared with the tumor from which the DNA was obtained as well as allogeneic determinants. DNA from a gp100(+) melanoma cell line was transduced into gp100(-) PCI-13/IL-2 cells (PCI-13/IL-2/DNA). A T cell line specific for the gp100 epitope responded to PCI-13/IL-2/DNA cells by IFN-gamma-secretion measured in enzyme-linked immunospot assays. The T cell line also recognized the gp100 epitope presented by dendritic cells that ingested PCI-13/IL-2/DNA cells, which had been induced by UVB irradiation to undergo apoptosis. After up-take and processing of apoptotic PCI-13/IL-2/DNA cells, the dendritic cells primed normal peripheral blood lymphocytes to generate effector T cells specific for the tumor donating the DNA. The results indicate that tumor epitopes encoded in such DNA are expressed in recipient cells and can induce tumor-specific T cells. The findings support translation of this vaccination strategy to a phase I trial in patients with cancer.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-10352316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-10559360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-10702485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-10754524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-10910067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-11007362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-11177563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-11286942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-1718596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-2137372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-2584720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-2766286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-7678351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-8145033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-8221636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-8986821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9032314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9368778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9408607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9415311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9510195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9570302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12080146-9614572
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SILV protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/gp100 Melanoma Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9415-20
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12080146-Antigen Presentation,
pubmed-meshheading:12080146-Apoptosis,
pubmed-meshheading:12080146-Cancer Vaccines,
pubmed-meshheading:12080146-Cell Line,
pubmed-meshheading:12080146-DNA, Neoplasm,
pubmed-meshheading:12080146-Dendritic Cells,
pubmed-meshheading:12080146-Epitopes,
pubmed-meshheading:12080146-Gene Expression,
pubmed-meshheading:12080146-Genes, Reporter,
pubmed-meshheading:12080146-Green Fluorescent Proteins,
pubmed-meshheading:12080146-HLA-A2 Antigen,
pubmed-meshheading:12080146-Humans,
pubmed-meshheading:12080146-Interleukin-2,
pubmed-meshheading:12080146-Luminescent Proteins,
pubmed-meshheading:12080146-Membrane Glycoproteins,
pubmed-meshheading:12080146-Neoplasm Proteins,
pubmed-meshheading:12080146-T-Lymphocytes,
pubmed-meshheading:12080146-Transduction, Genetic,
pubmed-meshheading:12080146-Tumor Cells, Cultured,
pubmed-meshheading:12080146-Vaccines, DNA,
pubmed-meshheading:12080146-gp100 Melanoma Antigen
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pubmed:year |
2002
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pubmed:articleTitle |
Human tumor-derived genomic DNA transduced into a recipient cell induces tumor-specific immune responses ex vivo.
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pubmed:affiliation |
Department of Pathology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA. whitesidetl@msx.upmc.edu
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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