Source:http://linkedlifedata.com/resource/pubmed/id/12077552
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
2002-6-21
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pubmed:abstractText |
Changes in ATP-induced increase in [Ca2+](i) during collecting duct ontogeny were studied in primary monolayer cultures of mouse ureteric bud (UB) and cortical collecting duct (CCD) cells by Fura-PE3 fluorescence ratio imaging. In UB (embryonic day E14 and postnatal day P1) the ATP-stimulated increase (EC(50) approximately 1 microM) in fluorescence ratio (DeltaR(ATP)) was independent of extracellular Ca2+ and insensitive to the P2 purinoceptor-antagonist suramin (1 mM). From day P7 onward when CCD morphogenesis had been completed DeltaR(ATP) increased and became dependent on extracellular Ca2+. This ATP-stimulated Ca2+ entry into CCD cells was non-capacitative and suramin (1 mM)-insensitive, but sensitive to nifedipine (30 microM) and enhanced by Bay K8644 (15 microM), a blocker and an agonist of L-type Ca2+ channels, respectively. Quantitative RT-PCR demonstrated similar mRNA expression of L-type Ca2+ channel alpha1-subunit, P2Y(1), P2Y(2), and P2X(4b) purinoceptors in UB and CCD monolayers while the abundance of P2X(4) mRNA increased with CCD morphogenesis. In conclusion, both embryonic and postnatal cells express probably P2Y(2)-stimulated Ca2+ release from intracellular stores. With development, the CCD epithelium acquires ATP-stimulated Ca2+ entry via L-type Ca2+ channels. This pathway might by mediated by the increasing expression of P2X(4)-receptors resulting in an increasing ATP-dependent membrane depolarization and activation of L-type Ca2+ channels.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic
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pubmed:status |
MEDLINE
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pubmed:issn |
1015-8987
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2002 S. Karger AG, Basel
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12077552-Adenosine Triphosphate,
pubmed-meshheading:12077552-Animals,
pubmed-meshheading:12077552-Calcium,
pubmed-meshheading:12077552-Calcium Channels, L-Type,
pubmed-meshheading:12077552-Calcium Signaling,
pubmed-meshheading:12077552-Cells, Cultured,
pubmed-meshheading:12077552-Epithelium,
pubmed-meshheading:12077552-Gene Expression Regulation, Developmental,
pubmed-meshheading:12077552-Kidney Tubules, Collecting,
pubmed-meshheading:12077552-Mice,
pubmed-meshheading:12077552-RNA, Messenger,
pubmed-meshheading:12077552-Receptors, Purinergic,
pubmed-meshheading:12077552-Ureter
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pubmed:year |
2002
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pubmed:articleTitle |
Ontogeny of purinergic receptor-regulated Ca2+ signaling in mouse cortical collecting duct epithelium.
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pubmed:affiliation |
Physiologisches Institut, Ludwig-Maximilians-Universität, Munich, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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