Source:http://linkedlifedata.com/resource/pubmed/id/12077260
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2002-6-21
|
| pubmed:abstractText |
Toxoplasma gondii is a parasite causing asymptomatic, persistent encephalitis. Protective CD4 and CD8 T cells are recruited to and accumulate in the brain in acute Toxoplasma encephalitis (TE), with slowly decreasing numbers in chronic TE. It is unclear how the size of the intracerebral T cell pool is regulated. Conceivably, permanent recruitment, proliferation, and apoptosis may be involved. We observed that in murine TE recruitment of T cells to the brain was terminated in chronic TE. In vivo 5-bromo-2'-deoxyuridine incorporation and in vitro T cell proliferation experiments revealed that intracerebral T cells did not proliferate, which was explained by the expression of the cell cycle inhibitors p21(Waf/cip1) and p27(Kip1) and the inhibitory activity of intracerebral F4/80(+) cells. TUNEL staining detected apoptotic T cells at low frequency corresponding to an increased expression of the anti-apoptotic molecules Bcl-2 and Bcl-x(L) and a reduced expression of the pro-apoptotic molecules Bad, Bax, and Fas ligand in CD4 and CD8 T cells. During progression from acute to chronic TE, both CD4 and CD8 T cells down-regulated CD45RB expression and expressed a differential pattern of cytokines. From these experiments it is concluded that the number of intracerebral T cells increases by recruitment of T cells during acute infection, whereas proliferation of intracerebral T cells does not play a role. In chronic TE, T cell recruitment is terminated, the phenotype of intracerebral T cells changes, and their number is gradually downsized by low level apoptosis, which, however, does not completely resolve the T cell infiltrates.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
169
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
315-22
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12077260-Animals,
pubmed-meshheading:12077260-Apoptosis,
pubmed-meshheading:12077260-Bromodeoxyuridine,
pubmed-meshheading:12077260-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12077260-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12077260-Cell Cycle,
pubmed-meshheading:12077260-Cell Division,
pubmed-meshheading:12077260-Cerebral Cortex,
pubmed-meshheading:12077260-Chemotaxis, Leukocyte,
pubmed-meshheading:12077260-Encephalitis,
pubmed-meshheading:12077260-Female,
pubmed-meshheading:12077260-Immunophenotyping,
pubmed-meshheading:12077260-Lymphocyte Activation,
pubmed-meshheading:12077260-Lymphocyte Count,
pubmed-meshheading:12077260-Mice,
pubmed-meshheading:12077260-T-Lymphocyte Subsets,
pubmed-meshheading:12077260-Time Factors,
pubmed-meshheading:12077260-Toxoplasmosis, Animal
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Phenotype and regulation of persistent intracerebral T cells in murine Toxoplasma encephalitis.
|
| pubmed:affiliation |
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Mannheim, Universität Heidelberg, Mannheim, Germany. dirk.schlueter@imh.ma.uni-heidelberg.de
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|