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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-6-21
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pubmed:abstractText |
The role of NKT cells on antitumor activity of CpG oligodeoxynucleotides (ODNs) was evaluated by peritumoral injections of CpG-ODNs in s.c. melanoma-bearing mice of strains differing in the number of NKT cells (athymic nude mice, recombination-activating gene(-/-)/transgenic V(alpha)14/Vbeta8.2 mice that generate NKT cells; J(alpha)281(-/-) mice and CD1(-/-) mice, which both have a strongly reduced number of NKT cells; and C57BL/6 wild-type mice). Tumor growth was significantly inhibited in strains enriched or depleted of NKT cells. The two murine strains having a reduced number of NKT cells differed significantly in the CpG-dependent tumor growth inhibition: in J(alpha)281(-/-) mice this inhibition was superimposable to that observed in C57BL/6 mice, while in CD1(-/-) mice the inhibition was dramatic. The increased tumor inhibition in CD1(-/-) correlated with a significantly higher ratio of IFN-gamma-IL-4 production in response to CpG as compared with C57BL/6 and J(alpha)281(-/-) mice. Experiments in which preparations of APCs and lymphocytes of the three strains were mixed showed that in the presence of APCs not expressing CD1, the production of CpG-ODN-induced type 1 cytokines was higher. Phenotype analysis of IFN-gamma- and IL-4-producing cells revealed that the differences between CD1(-/-) and C57BL/6 in the production of these two cytokines were mainly due to CD3(+) T lymphocytes. These data point to a regulatory role for the CD1 molecule in antitumor activity induced by danger signals, independently of V(alpha)14 NKT cells. The identification of a CD1-dependent suppressive subpopulation(s) might have important implications for the study of tolerance in the context of cancer, autoimmunity, and transplantation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/CPG-oligonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Rag2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
151-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12077240-Adjuvants, Immunologic,
pubmed-meshheading:12077240-Animals,
pubmed-meshheading:12077240-Antigen-Presenting Cells,
pubmed-meshheading:12077240-Antigens, CD1,
pubmed-meshheading:12077240-CpG Islands,
pubmed-meshheading:12077240-Cytokines,
pubmed-meshheading:12077240-DNA-Binding Proteins,
pubmed-meshheading:12077240-Homeodomain Proteins,
pubmed-meshheading:12077240-Immunophenotyping,
pubmed-meshheading:12077240-Injections, Intralesional,
pubmed-meshheading:12077240-Interferon-gamma,
pubmed-meshheading:12077240-Interleukin-4,
pubmed-meshheading:12077240-Killer Cells, Natural,
pubmed-meshheading:12077240-Melanoma, Experimental,
pubmed-meshheading:12077240-Mice,
pubmed-meshheading:12077240-Mice, Inbred BALB C,
pubmed-meshheading:12077240-Mice, Inbred C57BL,
pubmed-meshheading:12077240-Mice, Knockout,
pubmed-meshheading:12077240-Mice, Nude,
pubmed-meshheading:12077240-Mice, Transgenic,
pubmed-meshheading:12077240-Neoplasm Transplantation,
pubmed-meshheading:12077240-Oligodeoxyribonucleotides,
pubmed-meshheading:12077240-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12077240-T-Lymphocyte Subsets,
pubmed-meshheading:12077240-Up-Regulation
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pubmed:year |
2002
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pubmed:articleTitle |
Absence of the CD1 molecule up-regulates antitumor activity induced by CpG oligodeoxynucleotides in mice.
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pubmed:affiliation |
Molecular Targeting Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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