Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2002-6-21
pubmed:abstractText
Glutamate released onto retinal ON bipolar neurons binds to a metabotropic receptor to activate a heterotrimeric G-protein (G(o)) that ultimately closes a nonspecific cation channel. Signaling requires the alpha subunit (Galpha(o)), but its effector is unknown. Because Galpha(o) is transcribed into two splice variants (alpha(o1) and alpha(o2)) that differ in the key GTPase domain, the next step in elucidating this pathway was to determine which splice variant carries the signal. Here we show by reverse transcription-PCR and Western blots that retina expresses both splice variants. Furthermore, in situ hybridization and immunostaining on mouse retina deficient in one splice variant or the other show that both alpha(o1) and alpha(o2) are expressed by ON bipolar cells but that alpha(o1) is much more abundant. Finally, electroretinography performed on mice deficient for one splice variant or the other shows that the positive b-wave (response of ON bipolar cells to rod and cone input) requires alpha(o1) but not alpha(o2). Thus, the light response of the ON bipolar cell is probably carried by its strongly expressed splice variant, Galpha(o1).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4878-84
pubmed:dateRevised
2010-1-13
pubmed:meshHeading
pubmed-meshheading:12077185-Alternative Splicing, pubmed-meshheading:12077185-Amacrine Cells, pubmed-meshheading:12077185-Animals, pubmed-meshheading:12077185-GTP-Binding Protein alpha Subunits, Gi-Go, pubmed-meshheading:12077185-Heterotrimeric GTP-Binding Proteins, pubmed-meshheading:12077185-Kinetics, pubmed-meshheading:12077185-Light, pubmed-meshheading:12077185-Mice, pubmed-meshheading:12077185-Mice, Knockout, pubmed-meshheading:12077185-Photoreceptor Cells, Vertebrate, pubmed-meshheading:12077185-RNA, Messenger, pubmed-meshheading:12077185-Rats, pubmed-meshheading:12077185-Receptors, Kainic Acid, pubmed-meshheading:12077185-Retina, pubmed-meshheading:12077185-Retinal Cone Photoreceptor Cells, pubmed-meshheading:12077185-Retinal Rod Photoreceptor Cells, pubmed-meshheading:12077185-Vision, Ocular
pubmed:year
2002
pubmed:articleTitle
Light response of retinal ON bipolar cells requires a specific splice variant of Galpha(o).
pubmed:affiliation
Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. annu@retina.anatomy.upenn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't