Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2002-6-21
pubmed:databankReference
pubmed:abstractText
Transcriptional enhancers are short stretches of DNA that function to achieve highly specific patterns of gene expression. To identify the mechanisms by which enhancers achieve their specificity, we made use of an enhancer from the aboral ectoderm-specific spec2a gene of the sea urchin Strongylocentrotus purpuratus. The spec2a enhancer contains five cis-regulatory elements within 78 base pairs that interact with five distinct DNA-binding proteins to confer aboral ectoderm expression. Here, we present an analysis of the sea urchin CCAAT binding factor (CBF), which binds to a CCAAT motif within the spec2a enhancer. S. purpuratus CBF and SpOtx, a ubiquitously expressed factor, act together at closely placed cis-regulatory elements to mediate spec2a transcription in the ectoderm. SpCBF was the sole factor that bound to the spec2a CCAAT element, and two of the three subunits that make up the CBF holoprotein were cloned and shown to have high sequence conservation with their vertebrate orthologs. Based on its involvement in the regulation of several other sea urchin genes, SpCBF appears to be a major transcription factor in the sea urchin embryo for positive regulation of ectoderm gene expression. In addition to its role in vertebrate cell growth and proliferation, our results indicate that CBF also functions at the early stages of germ layer formation, namely ectoderm differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0301-4681
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-19
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12076338-Amino Acid Sequence, pubmed-meshheading:12076338-Animals, pubmed-meshheading:12076338-Binding Sites, pubmed-meshheading:12076338-CCAAT-Binding Factor, pubmed-meshheading:12076338-DNA, Complementary, pubmed-meshheading:12076338-Ectoderm, pubmed-meshheading:12076338-Embryo, Nonmammalian, pubmed-meshheading:12076338-Gene Expression Regulation, Developmental, pubmed-meshheading:12076338-Genes, Reporter, pubmed-meshheading:12076338-Humans, pubmed-meshheading:12076338-Molecular Sequence Data, pubmed-meshheading:12076338-Oligonucleotides, pubmed-meshheading:12076338-Protein Binding, pubmed-meshheading:12076338-Rats, pubmed-meshheading:12076338-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:12076338-Sea Urchins, pubmed-meshheading:12076338-Sequence Alignment, pubmed-meshheading:12076338-Transcriptional Activation
pubmed:year
2002
pubmed:articleTitle
Ectoderm gene activation in sea urchin embryos mediated by the CCAAT-binding factor.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Box 117, The University of Texas M. D. Anderson Cancer Center,1515 Holcombe Blvd., Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't