Linked Life Data

12072203

Source:http://linkedlifedata.com/resource/pubmed/id/12072203

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-6-19
pubmed:abstractText
Clear cell sarcoma (CCS), malignant melanoma of soft parts, is a rare malignant tumor with a poor prognosis. In this study, a CCS cell line, designated MP-CCS-SY, was established from a metastatic tumor of a 17-year-old Japanese girl that originated in the left Achilles tendon. A small number of melanosomes were detected in the cytoplasm by electron microscopy. The melanosomes immunoreacted with two melanoma-associated antibodies, HMB45 and Melan-A. A Western blot demonstrated the existence of a Melan-A antigen in this cell line. Although a t(12;22)(q13;q12), which is characteristic of CCS, was not identified by a chromosomal analysis with conventional banding techniques, fluorescence in situ hybridization analysis with painting probes of chromosomes 12 and 22 revealed the insertion of a chromosome 12 fragment into one of the long arms of chromosome 22. The chimeric EWS/ATF1 transcript was detected by the reverse transcriptase polymerase chain reaction. Extra copies and structural abnormalities of chromosome 8 were observed. Overexpression of c-myc mRNA was detected by Northern blot analysis and may have a role in malignant progression of CCS. The availability of this MP-CCS-SY cell line will help to understand the molecular biology of this malignancy and should be useful as a tool for developing an immunotherapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0165-4608
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-56
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12072203-Achilles Tendon, pubmed-meshheading:12072203-Adolescent, pubmed-meshheading:12072203-Antigens, Neoplasm, pubmed-meshheading:12072203-Chromosomes, Human, Pair 12, pubmed-meshheading:12072203-Chromosomes, Human, Pair 22, pubmed-meshheading:12072203-Chromosomes, Human, Pair 8, pubmed-meshheading:12072203-Disease Progression, pubmed-meshheading:12072203-Fatal Outcome, pubmed-meshheading:12072203-Female, pubmed-meshheading:12072203-Gene Expression Regulation, Neoplastic, pubmed-meshheading:12072203-Genes, myc, pubmed-meshheading:12072203-Humans, pubmed-meshheading:12072203-In Situ Hybridization, Fluorescence, pubmed-meshheading:12072203-MART-1 Antigen, pubmed-meshheading:12072203-Melanosomes, pubmed-meshheading:12072203-Mutagenesis, Insertional, pubmed-meshheading:12072203-Neoplasm Proteins, pubmed-meshheading:12072203-Oncogene Proteins, Fusion, pubmed-meshheading:12072203-Proto-Oncogene Proteins c-myc, pubmed-meshheading:12072203-RNA, Messenger, pubmed-meshheading:12072203-RNA, Neoplasm, pubmed-meshheading:12072203-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12072203-Sarcoma, Clear Cell, pubmed-meshheading:12072203-Soft Tissue Neoplasms, pubmed-meshheading:12072203-Transcription Factors, pubmed-meshheading:12072203-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Newly established clear cell sarcoma (malignant melanoma of soft parts) cell line expressing melanoma-associated Melan-A antigen and overexpressing C-MYC oncogene.
pubmed:affiliation
Department of Pediatrics, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki, Japan. moritake@fc.miyazaki-med.ac.jp
pubmed:publicationType
Journal Article, Case Reports, Research Support, Non-U.S. Gov't