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rdf:type |
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lifeskim:mentions |
umls-concept:C0006837,
umls-concept:C0007577,
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0055023,
umls-concept:C0086418,
umls-concept:C0205217,
umls-concept:C0225336,
umls-concept:C0887870,
umls-concept:C1171362,
umls-concept:C1414898,
umls-concept:C1515670,
umls-concept:C1519726,
umls-concept:C2700289
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pubmed:issue |
7
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pubmed:dateCreated |
2002-6-14
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pubmed:abstractText |
The signaling pathways triggered by adherence of Candida albicans to the host cells or extracellular matrix are poorly understood. We provide here evidence in C. albicans yeasts of a p105 focal adhesion kinase (Fak)-like protein (that we termed CaFak), antigenically related to the vertebrate p125Fak, and its involvement in integrin-like-mediated fungus adhesion to vitronectin (VN) and EA.hy 926 human endothelial cell line. Biochemical analysis with different anti-chicken Fak antibodies identified CaFak as a 105-kDa protein and immunofluorescence and cytofluorimetric analysis on permeabilized cells specifically stain C. albicans yeasts; moreover, confocal microscopy evidences CaFak as a cytosolic protein that colocalizes on the membrane with the integrin-like VN receptors upon yeast adhesion to VN. The protein tyrosine kinase (PTK) inhibitors genistein and herbimycin A strongly inhibited C. albicans yeast adhesion to VN and EA.hy 926 endothelial cells. Moreover, engagement of alpha v beta 3 and alpha v beta 5 integrin-like on C. albicans either by specific monoclonal antibodies or upon adhesion to VN or EA.hy 926 endothelial cells stimulates CaFak tyrosine phosphorylation that is blocked by PTK inhibitor. A role for CaFak in C. albicans yeast adhesion was also supported by the failure of VN to stimulate its tyrosine phosphorylation in a C. albicans mutant showing normal levels of CaFak and VNR-like integrins but displaying reduced adhesiveness to VN and EA.hy 926 endothelial cells. Our results suggest that C. albicans Fak-like protein is involved in the control of yeast cell adhesion to VN and endothelial cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10353874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10354709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10373530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10417206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10446041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10611323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10679059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10835423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-10972491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-11065354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-11114741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-11401997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-11562169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1385444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1429685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1528852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1594631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1700063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1741614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1900062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-1985087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-2030668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-6352500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7020114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7529097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7563001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7566154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7642098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7691609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7716514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-7822023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8060679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8105266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8118789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8454356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8552638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8617343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8794871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8856665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-8945550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9009353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9259554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9308185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9616126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9627997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9687528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9693367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9695829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12065524-9728395
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Protein-Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alphaV,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin beta Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin beta3,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/PTK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vitronectin,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Vitronectin,
http://linkedlifedata.com/resource/pubmed/chemical/integrin alphaVbeta5,
http://linkedlifedata.com/resource/pubmed/chemical/integrin beta5
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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