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pubmed-article:12065162pubmed:abstractTextWe assessed in vivo the anti-inflammatory activity of two Cat's claw bark extracts, by comparing a spray-dried hydroalcoholic extract against an aqueous freeze-dried extract, to determine which extract was more effective. We used the carrageenan-induced paw edema model in mice. In addition, to assess the molecular mechanism of action, we determined the inhibition of NF-kappa B through the Electrophoretic Mobility Shift Assay (EMSA) and the effects on cycloxygenase-1 and -2. Results showed that the anti-inflammatory activity was significantly higher using the hydroalcoholic compared with the aqueous extract (P<0.05). The extracts also showed little inhibitory activity on cyclooxygenase-1 and -2. It cannot be excluded that the slight inhibitory activity on DNA binding of NF-kappa B is due to cytotoxic effects.lld:pubmed
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pubmed-article:12065162pubmed:articleTitleAnti-inflammatory activity of two different extracts of Uncaria tomentosa (Rubiaceae).lld:pubmed
pubmed-article:12065162pubmed:affiliationMicrobiology Department, Faculty of Sciences and Philosophy, Universidad Peruana Cayetano Heredia, Apartado 4314, Lima 100, Peru. jaguilar@upch.edu.pelld:pubmed
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