|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
2002-6-13
|
|
pubmed:abstractText |
Site-selective dephosphorylation of receptor tyrosine kinases contributes to receptor regulation. The receptor-like protein tyrosine phosphatase DEP-1 site-selectively dephosphorylates the PDGF beta-receptor. DEP-1 dephosphorylation of original and chimeric phospho-peptides spanning the preferred pY1021 and the less preferred pY857 and pY562 sites was analyzed. Double substitutions of basic residues at -4 and +3 of pY857 and pY562 peptides improved affinity. Substitutions of single amino acids indicated preference for an acidic residue at position -1 and a preference against a basic residue at position +3. DEP-1 site-selective dephosphorylation of PDGF beta-receptor is thus determined by the primary sequence surrounding phosphorylation sites and involves interactions with residues spanning at least between positions -1 and +3.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
24
|
|
pubmed:volume |
517
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
27-31
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:12062403-Alanine,
pubmed-meshheading:12062403-Amino Acid Sequence,
pubmed-meshheading:12062403-Amino Acid Substitution,
pubmed-meshheading:12062403-Catalytic Domain,
pubmed-meshheading:12062403-Kinetics,
pubmed-meshheading:12062403-Lysine,
pubmed-meshheading:12062403-Molecular Sequence Data,
pubmed-meshheading:12062403-Phosphopeptides,
pubmed-meshheading:12062403-Phosphorylation,
pubmed-meshheading:12062403-Protein Tyrosine Phosphatases,
pubmed-meshheading:12062403-Receptor, Platelet-Derived Growth Factor beta,
pubmed-meshheading:12062403-Receptor-Like Protein Tyrosine Phosphatases, Class 3,
pubmed-meshheading:12062403-Substrate Specificity
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Primary sequence determinants responsible for site-selective dephosphorylation of the PDGF beta-receptor by the receptor-like protein tyrosine phosphatase DEP-1.
|
|
pubmed:affiliation |
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
|
|
pubmed:publicationType |
Journal Article
|
