Linked Life Data

12061863

Source:http://linkedlifedata.com/resource/pubmed/id/12061863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-6-13
pubmed:abstractText
Central nervous system progenitor cells that are self-renewing in culture and also differentiate under controlled conditions are potentially useful for developmental studies and for cell-based therapies. We characterized growth and plasticity properties and gene expression in a rat mesencephalic cell line, AF5, that was immortalized with an N-terminal fragment of SV40 large T (T155g). For over 150 population doublings in culture, the growth rate of AF5 cells remained steady, the cells remained responsive to bFGF, and telomerase activity and telomere lengths were unchanged. While karyotype analyses revealed some chromosomal abnormalities, these were also unchanged over time; additionally, no mutations in p53 gene sequences were found, and wild-type p53 activation was normal. AF5 cells produced PDGF, TGFbeta1, TGFbeta2, and bFGF. Similar to primary progenitor cells, AF5 cells retained their plasticity in culture; they could be propagated in an undifferentiated state as "neurospheres" in serum-free media or as adherent cultures in serum-containing media, and they differentiated when allowed to become confluent. Adherent subconfluent actively growing cultures expressed a marker for immature neurons, nestin, while few cells expressed the mature neuronal cell marker betaIII-tubulin. Confluent cultures ceased growing, developed differentiated morphologies, contained few or no nestin-expressing cells, and acquired betaIII-tubulin expression. Global gene expression was examined using a 15,000 gene microarray, comparing exponential growth with and without bFGF stimulation, and the differentiated state. The AF5 cell line exhibited stable genetic and growth properties over extended periods of time, while retaining the ability to differentiate in vitro. These data suggest that the AF5 cell line may be useful as an in vitro model system for studies of neural differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4886
pubmed:author
pubmed:copyrightInfo
(c) 2002 Elsevier Science (USA).
pubmed:issnType
Print
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12061863-Animals, pubmed-meshheading:12061863-Antigens, Polyomavirus Transforming, pubmed-meshheading:12061863-Cell Adhesion, pubmed-meshheading:12061863-Cell Differentiation, pubmed-meshheading:12061863-Cell Division, pubmed-meshheading:12061863-Cell Line, Transformed, pubmed-meshheading:12061863-Fibroblast Growth Factor 2, pubmed-meshheading:12061863-Gene Expression, pubmed-meshheading:12061863-Karyotyping, pubmed-meshheading:12061863-Mesencephalon, pubmed-meshheading:12061863-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12061863-Peptide Fragments, pubmed-meshheading:12061863-Phenotype, pubmed-meshheading:12061863-Platelet-Derived Growth Factor, pubmed-meshheading:12061863-Protein Structure, Tertiary, pubmed-meshheading:12061863-Rats, pubmed-meshheading:12061863-Stem Cells, pubmed-meshheading:12061863-Telomerase, pubmed-meshheading:12061863-Telomere, pubmed-meshheading:12061863-Transforming Growth Factor beta, pubmed-meshheading:12061863-Transforming Growth Factor beta1, pubmed-meshheading:12061863-Transforming Growth Factor beta2, pubmed-meshheading:12061863-Tumor Suppressor Protein p53
pubmed:year
2002
pubmed:articleTitle
AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
pubmed:affiliation
Cellular Neurobiology Research Branch, National Institute on Drug Abuse, 5500 Nathan Shock Drive, Baltimore, Maryland 21224, USA.
pubmed:publicationType
Journal Article