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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2002-6-12
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pubmed:abstractText |
Single nucleotide polymorphisms are implicated as having a significant role in regulating growth, development and, thereby, human health and disease. We have developed a method for identifying single nucleotide genetic alterations by combining hairpin-forming DNA probes and electrochemical detection of sandwich DNA hybridization. Incorporation of hairpin-forming competitor probes and the catalyzed reporter deposition amplification system further improves assay specificity by 7-fold and sensitivity by 100-fold. We have demonstrated that the system successfully identified the factor V Leiden mutations from human blood specimens.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-10026231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-10339560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11181995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11233601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11237011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11462842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11493890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-11701650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-1681511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-2192981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-2558138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-2984104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-6517326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-7877648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-8164741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-9415695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-9447593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-9508692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-9630890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12060693-9724323
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e55
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12060693-DNA Probes,
pubmed-meshheading:12060693-Electrochemistry,
pubmed-meshheading:12060693-Factor V,
pubmed-meshheading:12060693-Humans,
pubmed-meshheading:12060693-Immunoenzyme Techniques,
pubmed-meshheading:12060693-Nucleic Acid Conformation,
pubmed-meshheading:12060693-Oligonucleotide Probes,
pubmed-meshheading:12060693-Oligonucleotides,
pubmed-meshheading:12060693-Point Mutation,
pubmed-meshheading:12060693-Polymorphism, Single Nucleotide,
pubmed-meshheading:12060693-Sensitivity and Specificity,
pubmed-meshheading:12060693-Sequence Analysis, DNA
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pubmed:year |
2002
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pubmed:articleTitle |
An electrochemical detection scheme for identification of single nucleotide polymorphisms using hairpin-forming probes.
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pubmed:affiliation |
Mechanical and Aerospace Engineering Department, University of California-Los Angeles, Los Angeles, CA 90095-1597, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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