Source:http://linkedlifedata.com/resource/pubmed/id/12060463
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2002-6-12
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| pubmed:abstractText |
In order to investigate the mechanism by which CD4+ T cells and macrophages interact in the xenogeneic immune response, murine CD4+ T cells and macrophages were used as responder cells in culture with irradiated fetal pig spleen cells (FPSC) as pig xenogeneic stimulators. In this in vitro model, murine CD4+ T cells and macrophages were cultured individually, or together with FPSC. In addition, mouse CD4+ T cells were also cultured with autologous macrophages which were previously stimulated by FPSC. The cultured murine cells were analyzed for expression of CD4+ T cell and macrophage activation markers (cell surface markers and cytokines) as well as cytokine production. CD4+ T cells and macrophages cultured alone or together without FPSC showed unchanged low levels of expression of activation markers. Coculture of macrophages with FPSC and in the absence of CD4+ T cells induced increased expression levels of all the activation markers examined except B7.2 and ICAM-1. Addition of CD4+ T cells to the coculture further enhanced this up-regulation. Coculture of CD4+ T cells with FPSC-stimulated macrophages, but not naive macrophages, or FPSC alone, resulted in significantly increased numbers of CD4+ T cells coexpressing their activation markers, especially IFN-gamma and CD40L, and this expression was enhanced further by including FPSC in the coculture. The activation of both CD4+ T cells and macrophages in their coculture with FPSC was suppressed by neutralizing IFN-gamma but not IL-4. Our results demonstrated that interaction of CD4+ T cells and autologous macrophages was required for their optimal activation in response to pig xenogeneic stimulation. The mechanisms involved included cell-cell and/or cytokine interactions, and in particular IFN-gamma mediated communication was involved. Macrophages activated by pig cells in the absence of CD4+ T cells were able to activate naive CD4+ T cells, thus providing an important communication pathway between innate immune activation and a T cell mediated response in xenograft rejection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0908-665X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
268-76
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12060463-Animals,
pubmed-meshheading:12060463-Antibody Formation,
pubmed-meshheading:12060463-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12060463-Cell Membrane,
pubmed-meshheading:12060463-Cells, Cultured,
pubmed-meshheading:12060463-Cytokines,
pubmed-meshheading:12060463-DNA Primers,
pubmed-meshheading:12060463-Fetal Tissue Transplantation,
pubmed-meshheading:12060463-Flow Cytometry,
pubmed-meshheading:12060463-Interferon-gamma,
pubmed-meshheading:12060463-Interleukin-4,
pubmed-meshheading:12060463-Macrophage Activation,
pubmed-meshheading:12060463-Male,
pubmed-meshheading:12060463-Mice,
pubmed-meshheading:12060463-Mice, Inbred C57BL,
pubmed-meshheading:12060463-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12060463-Swine,
pubmed-meshheading:12060463-Transplantation, Heterologous
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
IFN-gamma but not IL-4 is important for mouse CD4+ T cell-mediated macrophage activation following their exposure to pig cells in vitro.
|
| pubmed:affiliation |
National Pancreas Transplant Unit, University of Sydney at Westmead Hospital, Westmead, NSW, Australia. philip_oconnell@wsahs.nsw.gov.au
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|