Linked Life Data

12060388

Source:http://linkedlifedata.com/resource/pubmed/id/12060388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-6-12
pubmed:abstractText
Mycosis fungoides (MF) is the most frequent manifestation of cutaneous T cell lymphoma but its cause and pathophysiology remain unclear. Because progression of lesions is characteristically slow, we hypothesized that mycosis fungoides originates from an accumulation of lymphocytes due to defective apoptosis of skin homing T lymphocytes. In this study, we investigate possible alterations of three molecules regulating apoptosis, i.e., Fas antigen, Bax, and p53, at the genomic level in skin lesions from 44 patients with MF, as Fas mediates one of two major pathways for apoptosis of activated T cells. Fas mutations were found in six patients using a polymerase chain reaction and single-strand conformational polymorphism method followed by cloning and sequencing of abnormal polymerase chain reaction products. The mutations predict for defective transmission of the death signal in three cases. Immunohistochemistry demonstrated the lack of Fas protein expression on dermal lymphocytes in one case with Fas gene mutation predicting for a truncated death domain, whereas Fas protein was expressed by dermal lymphocytes in the other investigated cases. By contrast, no mutations of Bax or p53 were found, whereas immunohistochemistry demonstrated increased p53 expression in the nucleus of basal keratinocytes above the neoplastic infiltrate in some MF cases. These results support the hypothesis that Fas defects may play a role in the pathogenesis of MF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
949-56
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12060388-Antigens, CD95, pubmed-meshheading:12060388-Apoptosis, pubmed-meshheading:12060388-DNA Primers, pubmed-meshheading:12060388-Exons, pubmed-meshheading:12060388-Humans, pubmed-meshheading:12060388-Immunohistochemistry, pubmed-meshheading:12060388-Mutation, pubmed-meshheading:12060388-Mycosis Fungoides, pubmed-meshheading:12060388-Polymerase Chain Reaction, pubmed-meshheading:12060388-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:12060388-Proto-Oncogene Proteins, pubmed-meshheading:12060388-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:12060388-Skin, pubmed-meshheading:12060388-Skin Neoplasms, pubmed-meshheading:12060388-T-Lymphocytes, pubmed-meshheading:12060388-Tumor Suppressor Protein p53, pubmed-meshheading:12060388-bcl-2-Associated X Protein
pubmed:year
2002
pubmed:articleTitle
Infrequent Fas mutations but no Bax or p53 mutations in early mycosis fungoides: a possible mechanism for the accumulation of malignant T lymphocytes in the skin.
pubmed:affiliation
Department of Pathology, Beth Israel Deaconess Medical Center-Harvard Medical School, Boston, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't