Linked Life Data

12055285

Source:http://linkedlifedata.com/resource/pubmed/id/12055285

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 6
pubmed:dateCreated
2002-6-10
pubmed:databankReference
pubmed:abstractText
Plasmid pIPO2 is a cryptic, conjugative, broad-host-range plasmid isolated from the wheat rhizosphere. It efficiently self-transfers between alpha, beta and gamma Proteobacteria and has a mobilizing/retromobilizing capacity for IncQ plasmids. The complete nucleotide sequence of pIPO2 is presented on the basis of its mini-Tn5::luxABtet-tagged derivative, pIPO2T. The pIPO2 sequence is 39815 bp long and contains at least 43 complete ORFs. Apart from a suite of ORFs with unknown function, all of the genes carried on pIPO2 are predicted to be involved in plasmid replication, maintenance and conjugative transfer. The overall organization of these genes is different from previously described plasmids, but is similar to the genetic organization seen in pSB102, a conjugative plasmid recently isolated from the bacterial community of the alfalfa rhizosphere. The putative conjugative transfer region of pIPO2 covers 23 kb and contains the genes required for DNA processing (Dtr) and mating pair formation (Mpf). The organization of these transfer genes in pIPO2 is highly similar to the genetic organization seen in the environmental plasmid pSB102 and in pXF51 from the plant pathogen Xylella fastidiosa. Plasmids pSB102 and pXF51 have recently been proposed to form a new family of environmental broad-host-range plasmids. Here it is suggested that pIPO2 is a new member of this family. The proposed Mpf system of pIPO2 shares high amino acid sequence similarity with equivalent VirB proteins from the type IV secretion system of Brucella spp. Sequence information was used to design primers specific for the detection of pIPO2. Environmental DNA from a range of diverse habitats was screened by PCR with these primers. Consistently positive signals for the presence of pIPO2 were obtained from a range of soil-related habitats, including the rhizospheres of young wheat plants, of field-grown oats and of grass (all gramineous plants), as well as from the rhizosphere of tomato plants. These data add to the growing evidence that plasmids carry advantageous genes with as yet undefined functions in plant-associated communities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1637-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12055285-Amino Acid Motifs, pubmed-meshheading:12055285-Amino Acid Sequence, pubmed-meshheading:12055285-Bacterial Proteins, pubmed-meshheading:12055285-Base Sequence, pubmed-meshheading:12055285-Chromosome Segregation, pubmed-meshheading:12055285-Conjugation, Genetic, pubmed-meshheading:12055285-DNA, Bacterial, pubmed-meshheading:12055285-DNA Replication, pubmed-meshheading:12055285-Molecular Sequence Data, pubmed-meshheading:12055285-Plant Roots, pubmed-meshheading:12055285-Plasmids, pubmed-meshheading:12055285-Promoter Regions, Genetic, pubmed-meshheading:12055285-Protein Sorting Signals, pubmed-meshheading:12055285-Sequence Analysis, DNA, pubmed-meshheading:12055285-Soil Microbiology, pubmed-meshheading:12055285-Species Specificity, pubmed-meshheading:12055285-Triticum
pubmed:year
2002
pubmed:articleTitle
The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere.
pubmed:affiliation
Zentrum für Genomforschung, Universität Bielefeld, D-33615 Bielefeld, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't