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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-6-10
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pubmed:abstractText |
Recent work identified ABCA1 as the major regulator of plasma HDL-cholesterol responsible for the removal of excess choline-phospholipids and cholesterol from peripheral cells and tissues. ABCA1 function may depend on the association with heteromeric proteins and to identify these candidates a human liver yeast two-hybrid library was screened with the carboxyterminal 144 amino acids of ABCA1. Beta2-syntrophin was found to interact with ABCA1 and the C-terminal five amino acids of ABCA1 proned to represent a perfect tail for binding to syntrophin PDZ domains. Immunoprecipitation further confirmed the association of ABCA1 and beta2-syntrophin and in addition utrophin, known to couple beta2-syntrophin and its PDZ ligands to the F-actin cytoskeleton, was identified as a constituent of this complex. ABCA1 in the plasmamembrane of human macrophages was found to be partially associated with Lubrol rafts and effluxed choline-phospholipids involve these microdomains. Beta2-syntrophin does not colocalize in these rafts indicating that beta2-syntrophin may participate in the retaining of ABCA1 in cytoplasmic vesicles and for the targeting of ABCA1 to plasmamembrane microdomains when ABCA1 is released from beta2-syntrophin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
759-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12054535-ATP-Binding Cassette Transporters,
pubmed-meshheading:12054535-Adult,
pubmed-meshheading:12054535-Amino Acid Sequence,
pubmed-meshheading:12054535-Cell Membrane,
pubmed-meshheading:12054535-Cells, Cultured,
pubmed-meshheading:12054535-Cytoplasmic Vesicles,
pubmed-meshheading:12054535-Cytoskeletal Proteins,
pubmed-meshheading:12054535-Dystrophin-Associated Proteins,
pubmed-meshheading:12054535-Fibroblasts,
pubmed-meshheading:12054535-Humans,
pubmed-meshheading:12054535-Macromolecular Substances,
pubmed-meshheading:12054535-Membrane Proteins,
pubmed-meshheading:12054535-Middle Aged,
pubmed-meshheading:12054535-Molecular Sequence Data,
pubmed-meshheading:12054535-Precipitin Tests,
pubmed-meshheading:12054535-Protein Structure, Tertiary,
pubmed-meshheading:12054535-Sequence Homology, Amino Acid,
pubmed-meshheading:12054535-Two-Hybrid System Techniques,
pubmed-meshheading:12054535-Utrophin
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pubmed:year |
2002
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pubmed:articleTitle |
The carboxyterminus of the ATP-binding cassette transporter A1 interacts with a beta2-syntrophin/utrophin complex.
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pubmed:affiliation |
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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