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rdf:type |
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lifeskim:mentions |
umls-concept:C0031727,
umls-concept:C0086418,
umls-concept:C0229671,
umls-concept:C0244988,
umls-concept:C0330390,
umls-concept:C0439851,
umls-concept:C1157202,
umls-concept:C1552596,
umls-concept:C1704675,
umls-concept:C1822702,
umls-concept:C1947931
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pubmed:issue |
4
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pubmed:dateCreated |
2002-6-10
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pubmed:abstractText |
Serum and glucocorticoid-inducible kinase-like kinase (SGKL) has been identified as a new integrator that decodes lipid signals produced by the activation of phosphoinositide 3-kinase (PI3K). SGKL is activated via its lipid-binding domain (phox homology domain) in response to PI3K signaling. However, downstream targets of SGKL as well as the role of SGKL as a mediator in PI3K signaling in human tissues remain to be established. In this study, we identified human glycogen synthase kinase 3 beta (GSK-3beta) as a specific interacting partner with SGKL in a yeast two-hybrid screening of human brain cDNA library. The association between these two proteins is confirmed independently in human embryonic kidney (HEK293) cells by co-immunoprecipitation. Furthermore, the kinase activity of wild-type SGKL was required for the in vitro phosphorylation of a GSK-3 crosstide fusion protein at serine-21/9 as demonstrated with a Phospho-GSK-3alpha/beta (Ser21/9) specific antibody. The present results provide strong evidences that SGKL could utilize GSK-3beta as a direct downstream target by phosphorylating GSK-3beta at serine-9.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1191-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12054501-Brain,
pubmed-meshheading:12054501-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:12054501-DNA, Complementary,
pubmed-meshheading:12054501-Gene Library,
pubmed-meshheading:12054501-Glycogen Synthase Kinase 3,
pubmed-meshheading:12054501-Glycogen Synthase Kinases,
pubmed-meshheading:12054501-Humans,
pubmed-meshheading:12054501-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12054501-Phosphorylation,
pubmed-meshheading:12054501-Plasmids,
pubmed-meshheading:12054501-Precipitin Tests,
pubmed-meshheading:12054501-Protein Binding,
pubmed-meshheading:12054501-Protein Structure, Tertiary,
pubmed-meshheading:12054501-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12054501-Serine,
pubmed-meshheading:12054501-Signal Transduction,
pubmed-meshheading:12054501-Two-Hybrid System Techniques
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pubmed:year |
2002
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pubmed:articleTitle |
Human serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK-3beta) at serine-9 through direct interaction.
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pubmed:affiliation |
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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