Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-6-10
pubmed:abstractText
The human cyclin Bl gene is cell cycle regulated with maximal activity during G(2)/M. We examined the role of histone deacetylation in cyclin Bl regulation using the histone deacetylase inhibitor trichostatin A (TSA). TSA treatment (100 ng/ml) of NIH3T3 cells containing the luciferase reporter construct pCycB(-287)-LUC caused an increase in promoter activity in G(0) and G(1) but no significant change in G(2). Removal of upstream sequences including an E-box and Sp1 site eliminated the TSA induced increase in G(0) and G(1), and caused a decrease in promoter activity during S and G(2). Promoter activity increased only 2-fold following TSA treatment of G(0) cells containing the construct pCycB(MUT-E-Box)-LUC with an E-box mutation, and a decrease in activity was detected during G(2). We conclude that histone deacetylation contributes to the repression of cyclin B1 expression in G(0) and G(1), and that this mechanism requires, in part, the E-box. TSA reduction of cyclin B1 promoter activity in G(2), however, involves sequences within the first 119 bp. A working model for cyclin B1 regulation is provided.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-9861
pubmed:author
pubmed:copyrightInfo
(c) 2002 Elsevier Science (USA).
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
401
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12054478-3T3 Cells, pubmed-meshheading:12054478-Acetylation, pubmed-meshheading:12054478-Animals, pubmed-meshheading:12054478-Cell Cycle, pubmed-meshheading:12054478-Cyclin B, pubmed-meshheading:12054478-Cyclin B1, pubmed-meshheading:12054478-Enzyme Inhibitors, pubmed-meshheading:12054478-G0 Phase, pubmed-meshheading:12054478-G1 Phase, pubmed-meshheading:12054478-G2 Phase, pubmed-meshheading:12054478-Genes, Regulator, pubmed-meshheading:12054478-Histone Deacetylase Inhibitors, pubmed-meshheading:12054478-Histones, pubmed-meshheading:12054478-Humans, pubmed-meshheading:12054478-Hydroxamic Acids, pubmed-meshheading:12054478-Mice, pubmed-meshheading:12054478-Models, Biological, pubmed-meshheading:12054478-Mutation, pubmed-meshheading:12054478-Promoter Regions, Genetic, pubmed-meshheading:12054478-S Phase, pubmed-meshheading:12054478-Up-Regulation
pubmed:year
2002
pubmed:articleTitle
Cell cycle specific changes in the human cyclin B1 gene regulatory region as revealed by response to trichostatin A.
pubmed:affiliation
Department of Biology, University of North Carolina at Greensboro, P.O. Box 26174, Greensboro, NC 27402-6174, USA. kskatula@uncg.edu
pubmed:publicationType
Journal Article