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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-6-10
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pubmed:abstractText |
In living organisms a permanent oxidation of protein oxidation occurs. The degradation of intracellular oxidized proteins is intensively studied, but knowledge about the fate of oxidatively modified extracellular proteins is still limited. We studied the fate of exogenously added oxidized proteins in microglial cells. Both primary microglial cells and RAW cells are able to remove added oxidized laminin and myelin basic protein from the extracellular environment. Moderately oxidized proteins are degraded most efficiently, whereas strongly oxidized proteins are taken up by the microglial cells without an efficient degradation. Activation of microglial cells enhances the selective recognition and degradation of moderately oxidized protein substrates by proteases. Inhibitor studies also revealed an involvement of the lysosomal and the proteasomal system in the degradation of extracellular proteins. These studies let us conclude that microglial cells are able to remove oxidized proteins from the extracellular environment in the brain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
400
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12054427-Animals,
pubmed-meshheading:12054427-Animals, Newborn,
pubmed-meshheading:12054427-Cells, Cultured,
pubmed-meshheading:12054427-Enzyme Inhibitors,
pubmed-meshheading:12054427-Extracellular Space,
pubmed-meshheading:12054427-Hydrogen Peroxide,
pubmed-meshheading:12054427-Laminin,
pubmed-meshheading:12054427-Lipopolysaccharides,
pubmed-meshheading:12054427-Macrophage Activation,
pubmed-meshheading:12054427-Macrophages,
pubmed-meshheading:12054427-Mice,
pubmed-meshheading:12054427-Microglia,
pubmed-meshheading:12054427-Microscopy, Fluorescence,
pubmed-meshheading:12054427-Myelin Basic Proteins,
pubmed-meshheading:12054427-Nitric Oxide,
pubmed-meshheading:12054427-Oxidants,
pubmed-meshheading:12054427-Oxidation-Reduction,
pubmed-meshheading:12054427-Rats,
pubmed-meshheading:12054427-Rats, Wistar,
pubmed-meshheading:12054427-Tetradecanoylphorbol Acetate,
pubmed-meshheading:12054427-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Degradation of oxidized extracellular proteins by microglia.
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pubmed:affiliation |
Neuroscience Research Center, Medical Faculty, Charité, Humboldt University Berlin, Schumannstrasse 20/21, D-10098 Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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