Linked Life Data

12050216

Source:http://linkedlifedata.com/resource/pubmed/id/12050216

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-6-6
pubmed:abstractText
VIP and pituitary adenylate cyclase-activating polypeptide (PACAP) are two regulatory peptides that possess remarkable amino acid sequence homology and act through common receptors, named PAC(1), VPAC(1), and VPAC(2). PAC(1) receptor is selective for PACAP, whereas VPAC(1) and VPAC(2) receptors bind both VIP and PACAP. We have investigated the expression and function of VIP, PACAP, and their receptors in the zona glomerulosa (ZG), zonae fasciculata and reticularis, and adrenal medulla (AM) of the human adrenal cortex. RT-PCR and RIA detected VIP and PACAP expression exclusively in AM cells. RT-PCR demonstrated the presence of PAC(1) mRNA only in AM and of VPAC(1) and VPAC(2) mRNAs in both ZG and AM cells. VIP and PACAP concentration-dependently increased aldosterone and catecholamine secretion from cultured ZG and AM cells. The catecholamine response to both peptides was higher than the aldosterone response, and the secretagogue action of PACAP was more intense than that of VIP. The aldosterone response of cultured ZG cells to VIP or PACAP was unaffected by the PAC(1) receptor antagonist PACAP-(6-38) (PAC(1)-A), but was significantly decreased by the VPAC(1) receptor antagonist [Ac-His(1),D-Phe(2),Lys(15),Arg(16)]VIP-(3-7),GH-releasing factor-(8-27)-NH(2) (VPAC(1)-A). The catecholamine response of cultured AM cells to VIP was lowered by VPAC(1)-A and unaffected by PAC(1)-A; conversely, the catecholamine response to PACAP was reduced by both PAC(1)-A and VPAC(1)-A. Simultaneous exposure to both antagonists did not abolish the catecholamine response to PACAP. Collectively, our findings allow us to conclude that in human adrenals 1) VIP and PACAP biosynthesis exclusively occurs in AM cells; 2) ZG cells are provided with functional VPAC(1) and VPAC(2) receptors, whose activation by VIP or PACAP elicits a moderate aldosterone response; 3) AM cells possess PAC(1), VPAC(1), and VPAC(2) receptors, whose activation evokes a marked catecholamine response; and 4) the catecholamine response to PACAP is more intense than that to VIP, because it is mediated by all subtypes of VIP/PACAP receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2575-80
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:12050216-Adrenal Cortex, pubmed-meshheading:12050216-Adrenal Glands, pubmed-meshheading:12050216-Adrenal Medulla, pubmed-meshheading:12050216-Adult, pubmed-meshheading:12050216-Aldosterone, pubmed-meshheading:12050216-Catecholamines, pubmed-meshheading:12050216-Humans, pubmed-meshheading:12050216-Middle Aged, pubmed-meshheading:12050216-Neuropeptides, pubmed-meshheading:12050216-Pituitary Adenylate Cyclase-Activating Polypeptide, pubmed-meshheading:12050216-Receptors, Pituitary Adenylate Cyclase-Activating..., pubmed-meshheading:12050216-Receptors, Pituitary Hormone, pubmed-meshheading:12050216-Receptors, Vasoactive Intestinal Peptide, pubmed-meshheading:12050216-Tissue Distribution, pubmed-meshheading:12050216-Vasoactive Intestinal Peptide, pubmed-meshheading:12050216-Zona Glomerulosa
pubmed:year
2002
pubmed:articleTitle
Expression and function of vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide, and their receptors in the human adrenal gland.
pubmed:affiliation
Department of Human Anatomy and Physiology, Section of Anatomy, University of Padua, Via Gabelli 65, I-35121 Padua, Italy.
pubmed:publicationType
Journal Article