Linked Life Data

12039748

Source:http://linkedlifedata.com/resource/pubmed/id/12039748

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-5-31
pubmed:abstractText
Historically, Vibrio parahaemolyticus infections have been characterized by sporadic cases caused by multiple, diverse serotypes. However, since 1996, V. parahaemolyticus serotype O3:K6 strains have been associated with several large-scale outbreaks of illness, suggesting the emergence of a "new" group of organisms with enhanced virulence. We have applied three different molecular subtyping techniques to identify an appropriate method for differentiating O3:K6 isolates from other serotypes. Pulsed-field gel electrophoresis (PFGE) following NotI digestion differentiated seven closely related subtypes among O3:K6 and related strains, which were distinct from PFGE patterns for non-O3:K6 isolates. Ribotyping and tdh sequencing were less discriminatory than PFGE, but further confirmed close genetic relationships among recent O3:K6 isolates. In vitro adherence and cytotoxicity studies with human epithelial cells showed that O3:K6 isolates exhibited statistically higher levels of adherence and cytotoxicity to host cells than non-O3:K6 isolates. Epithelial cell cytotoxicity patterns were determined with a lactate dehydrogenase release assay. At 3 h postinfection, high relative cytotoxicities (>50% maximum lactate dehydrogenase activity) were found among a greater proportion of recently isolated O3:K6 and closely related strains (75%) than among the non-O3:K6 isolates (23%). A statistically significant relationship between adherence and cytotoxicity suggests that the pathogenic potential of some isolates may be associated with increased adherence to epithelial cells. Our findings suggest that enhanced adherence and cytotoxicity may contribute to the apparent unique pathogenic potential of V. parahaemolyticus O3:K6 strains.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10364615, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10405387, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10655349, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10733249, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10746570, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10816461, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-10834969, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-11076722, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-11101606, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-11384535, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-11601716, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-3988703, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-6999994, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-7002028, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-8627070, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-8675334, http://linkedlifedata.com/resource/pubmed/commentcorrection/12039748-9399511
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2901-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Comparative phenotypic, molecular, and virulence characterization of Vibrio parahaemolyticus O3:K6 isolates.
pubmed:affiliation
Department of Food Science, Cornell University, Ithaca, New York 14853, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.