Linked Life Data

1203749

Source:http://linkedlifedata.com/resource/pubmed/id/1203749

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1976-3-15
pubmed:abstractText
In response to chemically-defined bone matrix gelatin (BMG) inside a diffusion chamber implanted in a muscle pouch, mesenchymal cells migrate directionally, aggregate and differentiate into new bone, on the outside of the chamber. BMG diffuses through double membranes 275 to 300 mum in thickness. The inner membrane of pore size is 0.025 mum and the outer membrane of pore size is 0.45 mum. The inner membrane is 1/20 the pore size and the combination is twice the thickness of membranes previously reported to transfer osteoinductive activity of living cells. Autoradiographs show 35S-cysteine-labelled BMG produces very high trans-membrane grain counts while 3H-proline labelled BMG produces very low transmembrane grain counts. Electron micrographs demonstrate that gelatin-derived, uranyl-acetate-stained fine granules interspersed with ruthenium red-staining coarse granules, diffuse through the membrane of 0.025 mum pore size from the inside out. Solitary pale-staining collagen fibrils, possibly formed in interstitial fluid by renaturation of BMG are found in the interior of the chamber and in the interior of the outer 0.45 mum but not the inner 0.025 mum pore size membrane. Densely-stained new bone collagen fiber bundles cover the outer membrane, fill the 0.45 mum subsurface pores for a depth of 0.20 to 30 mum, and thereby attach the new cartilage and bone deposits to the outer surface of the chamber. BMG powders solubilize rapidly in diffusion chambers and produce high yields of new bone. The relationship between denatured collagen and renatured gelatin fibrils in the process of transfer of the bone morphogen from BMG to mesenchymal cell receptors is an intriguing subject for further investigation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0008-0594
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-63
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Transmembrane bone matrix gelatin-induced differentiation of bone.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.