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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2002-8-5
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pubmed:abstractText |
Heparan sulfate (HS) regulates the kinetics of fibroblast growth factor 2 (FGF2)-stimulated intracellular signaling and differentially activates cell proliferation of cells expressing different FGF receptors (FGFRs). Evidence suggests that HS interacts with both FGFs and FGFRs to form active ternary signaling complexes. Here we compare the interactions of two FGFRs with HS. We show that the ectodomains of FGFR1 IIIc and FGFR2 IIIc exhibit specific interactions with different characteristics for both heparin and porcine mucosal HS. These glycans are both known to activate FGF signaling via these receptors. FGFR2 interacts with a higher apparent affinity than FGFR1 despite both involving 6-O-, 2-O-, and N-sulfates. FGFR1 and FGFR2 bind heparin with mean association rate constants of 1.9 x 10(5) and 2.1 x 10(6) m(-1)s(-1), respectively, and dissociation rate constants of 1.2 x 10(-2) and 2.7 x 10(-2) s(-1), respectively. These produced calculated affinities of 63 and 13 nm, respectively. Hence, FGFR1 and FGFR2 bind to heparin chains with markedly different kinetics and affinities. We propose a mechanistic model where the kinetic parameters of the HS/FGFR interaction are a key element regulating the formation of ternary complexes and the resulting FGF signaling outcomes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosaminoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Heparitin Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Fibroblast Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Fibroblast Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fibroblast Growth Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
28554-63
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12034712-Animals,
pubmed-meshheading:12034712-Binding, Competitive,
pubmed-meshheading:12034712-Biotinylation,
pubmed-meshheading:12034712-Cattle,
pubmed-meshheading:12034712-Dose-Response Relationship, Drug,
pubmed-meshheading:12034712-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12034712-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12034712-Fibroblast Growth Factors,
pubmed-meshheading:12034712-Glycosaminoglycans,
pubmed-meshheading:12034712-Heparin,
pubmed-meshheading:12034712-Heparitin Sulfate,
pubmed-meshheading:12034712-Kinetics,
pubmed-meshheading:12034712-Lung,
pubmed-meshheading:12034712-Models, Biological,
pubmed-meshheading:12034712-Protein Binding,
pubmed-meshheading:12034712-Protein Structure, Tertiary,
pubmed-meshheading:12034712-Receptor, Fibroblast Growth Factor, Type 1,
pubmed-meshheading:12034712-Receptor, Fibroblast Growth Factor, Type 2,
pubmed-meshheading:12034712-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:12034712-Receptors, Fibroblast Growth Factor,
pubmed-meshheading:12034712-Signal Transduction,
pubmed-meshheading:12034712-Swine
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pubmed:year |
2002
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pubmed:articleTitle |
Fibroblast growth factor receptors 1 and 2 interact differently with heparin/heparan sulfate. Implications for dynamic assembly of a ternary signaling complex.
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pubmed:affiliation |
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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