Source:http://linkedlifedata.com/resource/pubmed/id/12033497
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2002-5-29
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| pubmed:abstractText |
Interactions of serotonin (5-HT) with different specific 5-HT receptors that can coexist in the same blood vessel sometimes generate opposite effects. The aim of this study was to characterize the functional role of previously described mRNAs for 5-HT receptors in the rat aorta (5-HT(2A), 5-HT(2B), 5-HT(1B), 5-HT(7)) as well as to study the known 5-HT(2A) receptor-mediated constrictor response and investigate the influences of endothelium and preconstriction on the tissue in that response. A slight endothelium- and concentration-dependent relaxant effect was observed for 5-HT in aorta precontracted with either 5 microM phenylephrine (PE) or 1 microM prostaglandin F2alpha (PGF2alpha) in the presence of 0.3 microM ketanserin. EC50 values for 5-HT and alpha-methyl-5-HT relaxant responses after PE were 43.10 +/- 4.00 and 57.11 +/- 8.01 nM, respectively. pK(B) values for antagonists cyproheptadine and rauwolscine were 8.92 +/- 0.22 and 7.15 +/- 0.12, respectively. In nonprecontracted tissues, the contractile potency of 5-HT was higher in the absence of endothelium (EC50, degreesM): 2.60 +/- 0.28 and 4.12 +/- 0.21 in the absence and in the presence of endothelium, respectively. The differences were statistically significant (p<0.05). In precontracted tissues, the differences in EC50 values (2.22 +/- 0.40 and 4.65 +/- 0.60 microM without and with endothelium, respectively) were also statistically significant (p<0.05). pK(B) values for the 5-HT(2A) antagonist ketanserin were similar under all conditions tested. In conclusion, under our experimental conditions there are two functional 5-HT receptors in rat aorta: 5-HT(2A) contractile receptor in smooth muscle and a high-affinity relaxant receptor that mediates a very slight response and the pharmacology of which could be compatible with an endothelial 5-HT(2B) receptor.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyproheptadine,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost,
http://linkedlifedata.com/resource/pubmed/chemical/Ketanserin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT2A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0918-6158
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
584-90
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12033497-Algorithms,
pubmed-meshheading:12033497-Animals,
pubmed-meshheading:12033497-Aorta, Thoracic,
pubmed-meshheading:12033497-Cyproheptadine,
pubmed-meshheading:12033497-Dinoprost,
pubmed-meshheading:12033497-Ketanserin,
pubmed-meshheading:12033497-Male,
pubmed-meshheading:12033497-Rats,
pubmed-meshheading:12033497-Rats, Sprague-Dawley,
pubmed-meshheading:12033497-Receptor, Serotonin, 5-HT2A,
pubmed-meshheading:12033497-Receptors, Serotonin,
pubmed-meshheading:12033497-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12033497-Serotonin Antagonists
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| pubmed:year |
2002
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| pubmed:articleTitle |
Functional characterization of serotonin receptors in rat isolated aorta.
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| pubmed:affiliation |
Department of Pharmacology, School of Pharmacy, A Coruña, Spain.
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| pubmed:publicationType |
Journal Article
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