| pubmed-article:12032303 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C1419219 | lld:lifeskim |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C1424620 | lld:lifeskim |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C1709915 | lld:lifeskim |
| pubmed-article:12032303 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:12032303 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12032303 | pubmed:dateCreated | 2002-5-28 | lld:pubmed |
| pubmed-article:12032303 | pubmed:abstractText | TIP47 (tail-interacting protein of 47 kDa) binds to the cytoplasmic domains of the cation-dependent and cation-independent mannose 6-phosphate receptors (MPRs) and is required for their transport from endosomes to the trans-Golgi network in vitro and in living cells. TIP47 recognizes distinct determinants in the cytoplasmic domains of these two receptors, and its ability to bind to the cation-independent MPR is enhanced by the concomitant binding of the Rab9 GTPase. We show here that TIP47 residues 161-169 are essential, but likely not sufficient, for Rab9 binding. Mutation of these residues led to a significant decrease in Rab9 binding, but did not alter the global folding of the protein. The most impaired mutant was indistinguishable from wild-type TIP47 in its circular dichroism spectrum, and mutant proteins that showed decreased Rab9 binding retained full capacity to bind to MPR cytoplasmic domains. Closely related sequences in a related protein, adipophilin, did not confer Rab9 binding capacity to that protein. Partial proteolysis of TIP47 and TIP47 mutant proteins revealed subtle conformational differences, suggesting that residues 161-169 reside in a portion of TIP47 that is important for its conformation. These experiments reveal distinct binding domains for the Rab9 GTPase and MPR cytoplasmic domains in the cargo selection protein TIP47. | lld:pubmed |
| pubmed-article:12032303 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12032303 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12032303 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12032303 | pubmed:month | May | lld:pubmed |
| pubmed-article:12032303 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:12032303 | pubmed:author | pubmed-author:PfefferSuzann... | lld:pubmed |
| pubmed-article:12032303 | pubmed:author | pubmed-author:HannaJohnJ | lld:pubmed |
| pubmed-article:12032303 | pubmed:author | pubmed-author:CarrollKateK | lld:pubmed |
| pubmed-article:12032303 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12032303 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:12032303 | pubmed:volume | 99 | lld:pubmed |
| pubmed-article:12032303 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12032303 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12032303 | pubmed:pagination | 7450-4 | lld:pubmed |
| pubmed-article:12032303 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:meshHeading | pubmed-meshheading:12032303... | lld:pubmed |
| pubmed-article:12032303 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12032303 | pubmed:articleTitle | Identification of residues in TIP47 essential for Rab9 binding. | lld:pubmed |
| pubmed-article:12032303 | pubmed:affiliation | Department of Biochemistry, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305-5307, USA. | lld:pubmed |
| pubmed-article:12032303 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12032303 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| entrez-gene:10226 | entrezgene:pubmed | pubmed-article:12032303 | lld:entrezgene |
| entrez-gene:3482 | entrezgene:pubmed | pubmed-article:12032303 | lld:entrezgene |
| entrez-gene:9367 | entrezgene:pubmed | pubmed-article:12032303 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12032303 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12032303 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12032303 | lld:pubmed |
