Linked Life Data

12031456

Source:http://linkedlifedata.com/resource/pubmed/id/12031456

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-5-28
pubmed:abstractText
A complementary DNA (cDNA) encoding the eggshell zona radiata protein (RBTZR: AF407574) has been cloned from the liver of estradiol-17beta (E(2))-treated rainbow trout (Oncorhynchus mykiss) by reverse-transcriptase polymerase chain reaction (RT-PCR). A set of degenerate primers homologous to the highly conserved cysteine-rich region of the zona radiata protein gene from salmon, winter flounder, medaka and carp were used for the initial RT-PCR. The resulting PCR product was cloned, sequenced and identified as the Zrp gene fragment based on amino acid sequence similarities. Based on the Zrp sequence from the initial PCR, a pair of gene-sequence primers was designed for 3'- and 5'- random amplification of cDNA ends (RACE). Cloning and sequencing of RACE products showed a 1349-bp Zrp gene encoding a 403-amino acid protein with a theoretical molecular mass of approximately 45 kDa. Alignment of the deduced amino acid sequence reveals that RbtZR is similar to piscine and mammalian zona pellucida proteins. The RbtZR gene, together with the estrogen receptor (ER) and vitellogenin (Vtg) genes, was further characterized and comparatively studied for transcriptional and translational expression in xenoestrogen- (nonylphenol, NP) and E(2)-treated juvenile rainbow trout in a time-course experiment. Northern and slot blot analysis showed that the RbtZR mRNA was expressed, in parallel with the ER and Vtg mRNA, in both NP- and E(2)-treated juvenile rainbow trout. Indirect enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody raised against Atlantic salmon Zrp indicated the translational expression of RbtZR protein in blood plasma samples from NP- and E(2)-treated juvenile trout. The differential time-dependent transcriptional and translational expression and use of Zrp, ER and Vtg as sensitive biomarkers in environmental monitoring of endocrine disrupters in fish is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-26
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12031456-Amino Acid Sequence, pubmed-meshheading:12031456-Animals, pubmed-meshheading:12031456-Base Sequence, pubmed-meshheading:12031456-Cloning, Molecular, pubmed-meshheading:12031456-DNA, Complementary, pubmed-meshheading:12031456-DNA Primers, pubmed-meshheading:12031456-Egg Proteins, pubmed-meshheading:12031456-Egg Shell, pubmed-meshheading:12031456-Estradiol, pubmed-meshheading:12031456-Gene Expression Profiling, pubmed-meshheading:12031456-Gene Expression Regulation, pubmed-meshheading:12031456-Molecular Sequence Data, pubmed-meshheading:12031456-Oncorhynchus mykiss, pubmed-meshheading:12031456-Phenols, pubmed-meshheading:12031456-Phylogeny, pubmed-meshheading:12031456-Polymerase Chain Reaction, pubmed-meshheading:12031456-RNA, Messenger, pubmed-meshheading:12031456-Receptors, Estrogen, pubmed-meshheading:12031456-Vitellogenins
pubmed:year
2002
pubmed:articleTitle
Molecular cloning of rainbow trout (Oncorhynchus mykiss) eggshell zona radiata protein complementary DNA: mRNA expression in 17beta-estradiol- and nonylphenol-treated fish.
pubmed:affiliation
Great Lakes Institute for Environmental Research, University of Windor, 401 Sunset Avenue, Windsor, Ontario N9B 3P4, Canada. augustine.arukwe@mbi.uib.no
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't