Linked Life Data

12029356

Source:http://linkedlifedata.com/resource/pubmed/id/12029356

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-5-24
pubmed:abstractText
To characterize the coding-sequence divergence of closely related genomes, we compared DNA sequence divergence between sequences from a Brassica rapa ssp. pekinensis EST library isolated from flower buds and genomic sequences from Arabidopsis thaliana. The specific objectives were (i) to determine the distribution of and relationship between K(a) and K(s), (ii) to identify genes with the lowest and highest K(a): K(s) values, and (iii) to evaluate how codon usage has diverged between two closely related species. We found that the distribution of K(a): K(s) was unimodal, and that substitution rates were more variable at nonsynonymous than synonymous sites, and detected no evidence that K(a) and K(s) were positively correlated. Several genes had K(a): K(s) values equal to or near zero, as expected for genes that have evolved under strong selective constraint. In contrast, there were no genes with K(a): K(s) >1 and thus we found no strong evidence that any of the 218 sequences we analyzed have evolved in response to positive selection. We detected a stronger codon bias but a lower frequency of GC at synonymous sites in A. thaliana than B. rapa. Moreover, there has been a shift in the profile of most commonly used synonymous codons since these two species diverged from one another. This shift in codon usage may have been caused by stronger selection acting on codon usage or by a shift in the direction of mutational bias in the B. rapa phylogenetic lineage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2844
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
746-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Coding sequence divergence between two closely related plant species: Arabidopsis thaliana and Brassica rapa ssp. pekinensis.
pubmed:affiliation
Department of Ecology and Evolutionary Biology, University of California at Irvine, Irvine, CA 92664, USA. ptiffin@umn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.