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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5572
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pubmed:dateCreated |
2002-5-24
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pubmed:abstractText |
Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acyl-tRNA Synthetases,
http://linkedlifedata.com/resource/pubmed/chemical/Anticodon,
http://linkedlifedata.com/resource/pubmed/chemical/Archaeal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/Codon, Terminator,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Archaeal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/dimethylamine-coenzyme M...,
http://linkedlifedata.com/resource/pubmed/chemical/monomethylamine methyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/pyrrolysine
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
24
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1459-62
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:12029131-Amino Acid Sequence,
pubmed-meshheading:12029131-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:12029131-Anticodon,
pubmed-meshheading:12029131-Archaeal Proteins,
pubmed-meshheading:12029131-Base Sequence,
pubmed-meshheading:12029131-Catalytic Domain,
pubmed-meshheading:12029131-Codon,
pubmed-meshheading:12029131-Codon, Terminator,
pubmed-meshheading:12029131-Kinetics,
pubmed-meshheading:12029131-Lysine,
pubmed-meshheading:12029131-Methanosarcina barkeri,
pubmed-meshheading:12029131-Methyltransferases,
pubmed-meshheading:12029131-Molecular Sequence Data,
pubmed-meshheading:12029131-Nucleic Acid Conformation,
pubmed-meshheading:12029131-Protein Biosynthesis,
pubmed-meshheading:12029131-RNA, Archaeal,
pubmed-meshheading:12029131-RNA, Transfer,
pubmed-meshheading:12029131-Recombinant Proteins,
pubmed-meshheading:12029131-Sequence Alignment
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pubmed:year |
2002
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pubmed:articleTitle |
Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.
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pubmed:affiliation |
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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