Linked Life Data

12028379

Source:http://linkedlifedata.com/resource/pubmed/id/12028379

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-5-24
pubmed:abstractText
The Staphylococcus aureus DtxR-like protein, MntR, controls expression of the mntABC and mntH genes, which encode putative manganese transporters. Mutation of mntABC produced a growth defect in metal-depleted medium and increased sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted from diminished uptake of manganese and were rescued by the addition of excess Mn(II). Resistance to superoxide was incompletely rescued by Mn(II) for STE035 (mntA mntH), and the strain had reduced virulence in a murine abscess model of infection. Expression of mntABC was repressed by Mn(II) in an MntR-dependent manner, which contrasts with the expression of mntH that was not repressed in elevated Mn(II) and was decreased in an mntR mutant. This demonstrates that MntR acts as a negative and positive regulator of these loci respectively. PerR, the peroxide resistance regulon repressor, acts with MntR to control the expression of mntABC and manganese uptake. The expression of the PerR-regulated genes, katA (catalase), ftn (ferritin) and fur (ferric uptake regulator), was diminished in STE031 (mntR) when grown in excess Mn(II). Therefore, the control of Mn(II)-regulated members of the PerR regulon and the Fur protein is modulated by MntR through its control of Mn(II) uptake. The co-ordinated regulation of metal ion homeostasis and oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1269-86
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12028379-Animals, pubmed-meshheading:12028379-Bacterial Proteins, pubmed-meshheading:12028379-Base Sequence, pubmed-meshheading:12028379-Endothelium, Vascular, pubmed-meshheading:12028379-Gene Expression Regulation, Bacterial, pubmed-meshheading:12028379-Genes, Bacterial, pubmed-meshheading:12028379-Genes, Reporter, pubmed-meshheading:12028379-Herbicides, pubmed-meshheading:12028379-Homeostasis, pubmed-meshheading:12028379-Humans, pubmed-meshheading:12028379-Manganese, pubmed-meshheading:12028379-Mice, pubmed-meshheading:12028379-Mice, Inbred BALB C, pubmed-meshheading:12028379-Molecular Sequence Data, pubmed-meshheading:12028379-Paraquat, pubmed-meshheading:12028379-Promoter Regions, Genetic, pubmed-meshheading:12028379-Recombinant Fusion Proteins, pubmed-meshheading:12028379-Regulon, pubmed-meshheading:12028379-Repressor Proteins, pubmed-meshheading:12028379-Staphylococcus aureus, pubmed-meshheading:12028379-Superoxides, pubmed-meshheading:12028379-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake.
pubmed:affiliation
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't