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rdf:type |
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lifeskim:mentions |
umls-concept:C0007616,
umls-concept:C0007634,
umls-concept:C0026764,
umls-concept:C0032520,
umls-concept:C0205556,
umls-concept:C0392762,
umls-concept:C0882849,
umls-concept:C1511790,
umls-concept:C1609982,
umls-concept:C2347644,
umls-concept:C2349974
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pubmed:issue |
3
|
|
pubmed:dateCreated |
2002-5-24
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|
pubmed:abstractText |
The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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|
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0007-1048
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pubmed:author |
pubmed-author:BoccadoroMarioM,
pubmed-author:CastellinoClaudiaC,
pubmed-author:CorradiniPaoloP,
pubmed-author:LadettoMarcoM,
pubmed-author:LocatelliGiovanniG,
pubmed-author:LussoPaoloP,
pubmed-author:MalnatiMauroM,
pubmed-author:MilaniRaffaellaR,
pubmed-author:OmedèPaolaP,
pubmed-author:PerfettiVittorioV,
pubmed-author:PileriAlessandroA,
pubmed-author:VillaChiaraC,
pubmed-author:VoenaClaudiaC,
pubmed-author:ZapponeElisabettaE
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|
pubmed:issnType |
Print
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pubmed:volume |
117
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
642-5
|
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12028036-Aged,
pubmed-meshheading:12028036-Antigens, CD34,
pubmed-meshheading:12028036-Bone Marrow Purging,
pubmed-meshheading:12028036-Female,
pubmed-meshheading:12028036-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:12028036-Humans,
pubmed-meshheading:12028036-Immunomagnetic Separation,
pubmed-meshheading:12028036-Leukapheresis,
pubmed-meshheading:12028036-Male,
pubmed-meshheading:12028036-Middle Aged,
pubmed-meshheading:12028036-Multiple Myeloma,
pubmed-meshheading:12028036-Neoplasm, Residual,
pubmed-meshheading:12028036-Polymerase Chain Reaction
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|
pubmed:year |
2002
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|
pubmed:articleTitle |
Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system.
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|
pubmed:affiliation |
Department of Haematology, Laboratory of Molecular Haematology, Istituto H.S. Raffaele, Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|
