Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2002-8-21
pubmed:abstractText
Although human MUC3 and rodent Muc3 are both membrane-associated intestinal mucins, the present study has explored the possibility that rodent Muc3 might exist in soluble as well as membrane forms. No evidence was obtained for the existence of soluble splice variants; however, experiments with heterologous cells transfected with cDNA encoding the 381-residue C-terminal domain of rodent Muc3 showed that a definitive proteolytic cleavage occurs during processing in the endoplasmic reticulum. The products consisted of a V5-tagged 30 kDa extracellular glycopeptide and a Myc-tagged 49 kDa membrane-associated glycopeptide. Throughout their cellular transport to the plasma membrane, the two fragments remained associated by non-covalent SDS-sensitive interactions. Site-specific mutagenesis pinpointed the need for glycine and serine residues in the cleavage sequence Leu-Ser-Lys-Gly-Ser-Ile-Val-Val, which is localized between the two epidermal-growth-factor-like motifs of the mucin. A similar cleavage sequence (Phe-Arg-Pro-Gly downward arrow Ser-Val-Val-Val, where downward arrow signifies the cleavage site) has been reported in human MUC1 and analogous sites are present in human MUC3, MUC12 and MUC17. Thus early proteolytic cleavage may be a conserved characteristic of many membrane-associated mucins, possibly as a prelude to later release of their large extracellular domains at cell surfaces.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10024507, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10197628, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10389761, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10405327, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10463611, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10512748, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10542055, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10764832, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10806399, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10880978, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10908374, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-10920259, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11135323, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11278439, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11341784, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11352827, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11559653, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11847293, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-11855812, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-1379596, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-1478934, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-1556125, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-1599444, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-2111320, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-2208144, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-3731108, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-3902893, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8049433, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8407976, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8910003, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8910427, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8912645, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-8969211, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-9180140, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-9188795, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-9407114, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-9480884, http://linkedlifedata.com/resource/pubmed/commentcorrection/12027806-9494100
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
366
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
623-31
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:12027806-Humans, pubmed-meshheading:12027806-Animals, pubmed-meshheading:12027806-Mice, pubmed-meshheading:12027806-Rodentia, pubmed-meshheading:12027806-Mucins, pubmed-meshheading:12027806-Intestinal Mucosa, pubmed-meshheading:12027806-Genetic Variation, pubmed-meshheading:12027806-Endoplasmic Reticulum, pubmed-meshheading:12027806-Amino Acid Sequence, pubmed-meshheading:12027806-Binding Sites, pubmed-meshheading:12027806-Microvilli, pubmed-meshheading:12027806-Hydrolysis, pubmed-meshheading:12027806-Cercopithecus aethiops, pubmed-meshheading:12027806-Transfection, pubmed-meshheading:12027806-Recombinant Proteins, pubmed-meshheading:12027806-COS Cells, pubmed-meshheading:12027806-Mutagenesis, Site-Directed, pubmed-meshheading:12027806-Alternative Splicing, pubmed-meshheading:12027806-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12027806-Mucin-3
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