rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2002-5-23
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pubmed:abstractText |
Sambucus nigra agglutinin I (SNA-I) is a type 2 ribosome-inactivating protein. Site-directed mutagenesis was used to mimic the conversion of the highly active B-chain of fruit-specific SNA (SNA-If) into the completely inactive B-chain of the closely related and naturally occurring loss-of-activity mutant called S. nigra agglutinin lectin-related protein. In the first mutant SNA-If-M1 the high-affinity site 2 of SNA-If was disrupted by replacing the presumed critical residue Asp231 with Glu231. In the double mutant SNA-If-M2, site 1 of SNA-If-M1 was also disrupted by substituting the presumed critical residue Asn48 with Ser48. The parent type 2 ribosome-inactivating protein and both mutants were expressed in Nicotiana tabacum Samsun NN and the recombinant proteins were purified and analysed. Recombinant SNA-If agglutinated rabbit erythrocytes equally well as SNA-If, but both mutants were completely inactive in this test. Binding assays to immobilized galactose and fetuin revealed that the mutation Asp231-->Glu231 reduces the affinity of the B-chain for galactose and fetuin by more than 50%. Furthermore, the introduction of the second mutation Asn48-->Ser48 reduces the binding activity to less than 20% of the original activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-11161044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-11427481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-11513087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-12127253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-1369289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-1438186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-1463855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-1718752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-1881880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-2441069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-3536898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-3571242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-4093443,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-8631319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-8647092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-8738213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-8741988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023903-9079659
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0264-6021
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
364
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
587-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12023903-Acetylgalactosamine,
pubmed-meshheading:12023903-Amino Acid Sequence,
pubmed-meshheading:12023903-Carbohydrate Metabolism,
pubmed-meshheading:12023903-Lectins,
pubmed-meshheading:12023903-Molecular Sequence Data,
pubmed-meshheading:12023903-Mutagenesis, Site-Directed,
pubmed-meshheading:12023903-Plant Lectins,
pubmed-meshheading:12023903-Recombinant Proteins,
pubmed-meshheading:12023903-Ribosomes,
pubmed-meshheading:12023903-Sambucus nigra,
pubmed-meshheading:12023903-Sequence Homology, Amino Acid
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pubmed:year |
2002
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pubmed:articleTitle |
Mutational analysis of the carbohydrate-binding activity of the NeuAc(alpha-2,6)Gal/GalNAc-specific type 2 ribosome-inactivating protein from elderberry (Sambucus nigra) fruits.
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pubmed:affiliation |
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 Leuven, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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