Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-5-22
pubmed:abstractText
Myristoyl switch is a feature of several peripheral membrane proteins involved in signal transduction pathways. This unique molecular property is best illustrated by the "Ca(2+)-myristoyl switch" of recoverin, which is a Ca(2+)-binding protein present in retinal rod cells of vertebrates. In this transduction pathway, the Ca(2+)-myristoyl switch acts as a calcium sensor involved in cell recovery from photoactivation. Ca(2+) binding by recoverin induces the extrusion of its myristoyl group to the solvent, which leads to its translocation from cytosol to rod disk membranes. Force spectroscopy, based on atomic force microscope (AFM) technology, was used to determine the extent of membrane binding of recoverin in the absence and presence of calcium, and to quantify this force of binding. An adhesion force of 48 +/- 5 pN was measured between recoverin and supported phospholipid bilayers in the presence of Ca(2+). However, no binding was observed in the absence of Ca(2+). Experiments with nonmyristoylated recoverin confirmed these observations. Our results are consistent with previously measured extraction forces of lipids from membranes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10074420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10446384, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10500301, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10557292, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10753119, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10769056, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10854320, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-10940241, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-11015193, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-11115393, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-1385864, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-1386601, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-1454850, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-1672047, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-2406721, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-2813338, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-3978184, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7527558, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7547868, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7608179, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7629115, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7630423, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7667880, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7681327, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7919779, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7939660, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7947714, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-7961759, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8029471, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8075075, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8153628, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8242744, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8392055, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8399188, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8430337, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8461139, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8519970, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8521974, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-8537345, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9148804, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9296500, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9315839, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9533704, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9545566, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9600991, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9603523, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9665732, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9838145, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9931348, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023256-9973570
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3343-50
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
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