Linked Life Data

12021913

Source:http://linkedlifedata.com/resource/pubmed/id/12021913

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-5-21
pubmed:abstractText
Teleost fish are known to develop small populations of muscle fibres that are assumed to be tonic in nature although their contractile properties and many other characteristics remain unknown. Here we attempt to resolve some of the ambiguity and confusions surrounding the definition and functional role of tonic fibres in teleosts and provide new information on their ontogeny. We investigate the differentiation of tonic muscle fibres in three species of cyprinid fish using electron microscopy, histochemistry, immunohistochemistry and in situ hybridization. The fine structure of the fibres defined as tonic in the larvae used in this study complies with patterns known from studies in teleost adults. This allows formal definition of tonic fibres in cyprinid larvae. The tonic fibres may be recognized by a variety of features: (1) by their characteristic position along the medial confines of the red muscle insertion at the horizontal septum, (2) their fine structure, including solid clusters of irregularly cleaved myofibrils, thick and wavy Z-lines, and T-tubules at the A-band/I-band transitions, (3) their histochemical features, specifically weak but obvious staining for mATPase after alkaline preincubation, and lack of SDH activity in the more advanced larval stages, (4) their unique immunological properties, being the only fibre type in the myotome that reacts with a serum against chicken tonic myosin (anti- T2). Expression of tonic characters usually begins within a few fibres in the dorsal domain of the superficial red muscle insertion at the horizontal septum and hence involves a high degree of dorso-ventral polarity. The present evidence indicates that tonic fibres arise from separate myogenic stem cells rather than by transdifferentiation from existing red fibres. First appearance of tonic fibres during ontogeny correlates closely with the onset of free swimming and exogeneous feeding. We use this fact to argue that tonic fibres are probably a prerequisite for efficient locomotory control during prey capture, shoaling and predator avoidance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0340-2061
pubmed:author
pubmed:issnType
Print
pubmed:volume
205
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12021913-Animals, pubmed-meshheading:12021913-Ca(2+) Mg(2+)-ATPase, pubmed-meshheading:12021913-Cell Differentiation, pubmed-meshheading:12021913-Cyprinidae, pubmed-meshheading:12021913-DNA-Binding Proteins, pubmed-meshheading:12021913-Embryo, Nonmammalian, pubmed-meshheading:12021913-Embryonic and Fetal Development, pubmed-meshheading:12021913-Feeding Behavior, pubmed-meshheading:12021913-Immunohistochemistry, pubmed-meshheading:12021913-In Situ Hybridization, pubmed-meshheading:12021913-Muscle, Skeletal, pubmed-meshheading:12021913-Muscle Fibers, Slow-Twitch, pubmed-meshheading:12021913-MyoD Protein, pubmed-meshheading:12021913-Myogenic Regulatory Factors, pubmed-meshheading:12021913-RNA, Messenger, pubmed-meshheading:12021913-Succinate Dehydrogenase, pubmed-meshheading:12021913-Swimming, pubmed-meshheading:12021913-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
Tonic fibres in axial muscle of cyprinid fish larvae: their definition, possible origins and functional importance.
pubmed:affiliation
University of Salzburg, Institute of Zoology, Hellbrunnerstrasse 34, 5020 Salzburg, Austria. walter.stoiber@sbg.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't