Source:http://linkedlifedata.com/resource/pubmed/id/12021172
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2002-5-21
|
| pubmed:abstractText |
A novel first exon, E1(4), whose sequence was distinct from those of the three known first exons, E1(1), E1(2), and E1(3), of the rat PRL receptor (PRL-R) gene was identified by cDNA cloning for the 5'-end region of PRL-R mRNA expressed in the rat brain. Sequence analysis revealed the presence of two different length E1(4) cDNAs. The longer cDNA contained the 243-bp E1(4) sequence, and the shorter cDNA lacked the 139-bp sequence at the 5'-end of the longer one. Neither E1(4) cDNA has a second exon sequence, indicating that the E1(4) first exon is extensively spliced to the third exon. E1(4)-containing PRL-R mRNAs were detected only in the brain by RT-PCR and ribonuclease protection assay. The longer E1(4) mRNA was expressed as the major PRL-R mRNA species in the brain and was greatly increased in pregnant (d 18) and lactating (d 5) rats. A genomic clone containing the E1(4) first exon together with its 5'- and 3'-flanking regions was isolated from a rat kidney genomic library. Ribonuclease protection assay revealed that the position corresponding to the 5'-end of the shorter E1(4) cDNA is the major transcription start point for the E1(4) exon. The 5'-flanking region of E1(4) contained a TATA box-like element 23 bp upstream of the major transcription start point. Other putative transcription factor-binding sites, such as CCAAT, Sp1, and glucocorticoid-responsive elements, were observed at further upstream regions. These results suggest that PRL-R gene expression in rat brain is controlled by the promoter for the E1(4) first exon.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
143
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2080-4
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12021172-Animals,
pubmed-meshheading:12021172-Base Sequence,
pubmed-meshheading:12021172-Brain Chemistry,
pubmed-meshheading:12021172-Cloning, Molecular,
pubmed-meshheading:12021172-Embryonic and Fetal Development,
pubmed-meshheading:12021172-Exons,
pubmed-meshheading:12021172-Female,
pubmed-meshheading:12021172-Male,
pubmed-meshheading:12021172-Molecular Sequence Data,
pubmed-meshheading:12021172-Nuclease Protection Assays,
pubmed-meshheading:12021172-Pregnancy,
pubmed-meshheading:12021172-RNA, Messenger,
pubmed-meshheading:12021172-Rats,
pubmed-meshheading:12021172-Rats, Sprague-Dawley,
pubmed-meshheading:12021172-Receptors, Prolactin,
pubmed-meshheading:12021172-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12021172-Tissue Distribution,
pubmed-meshheading:12021172-Transcription, Genetic
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Identification of a novel first exon of prolactin receptor gene expressed in the rat brain.
|
| pubmed:affiliation |
Department of Biochemistry, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|