Linked Life Data

12020947

Source:http://linkedlifedata.com/resource/pubmed/id/12020947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-5-21
pubmed:abstractText
DNA methylation patterns change with age in a complex fashion, typically with an overall decrease in genomic deoxymethylcytosine (d(m)C) content, but with local increases in some promoters that contain GC-rich sequences known as CpG islands. While the consequences of age-dependent CpG island methylation have recently been studied in organs such as the colon, less is known about the functional significance of the progressive hypomethylation of promoters lacking CpG islands, and the significance of age-dependent changes in T cell DNA methylation is completely unexplored. We asked if age-dependent DNA hypomethylation might contribute to overexpression of the T cell ITGAL gene, which encodes CD11a, a subunit of LFA-1. CD11a mRNA increased with age as well as with experimentally induced DNA hypomethylation. This increase correlated with hypomethylation of sequences flanking the ITGAL promoter in vitro and in aging. 'Patch' methylation of the region suppressed promoter function. DNA methyltransferases 1 and 3a also decreased with aging. These results indicate that hypomethylation of regions flanking the ITGAL promoter may increase CD11a expression, and suggest that age-dependent hypomethylation of promoters lacking CpG islands, perhaps due to decreased DNA methyltransferase expression, may be one mechanism contributing to increased T cell gene expression with aging.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0047-6374
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1257-68
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12020947-Adult, pubmed-meshheading:12020947-Aged, pubmed-meshheading:12020947-Aging, pubmed-meshheading:12020947-Bacterial Proteins, pubmed-meshheading:12020947-DNA, pubmed-meshheading:12020947-DNA Methylation, pubmed-meshheading:12020947-DNA Modification Methylases, pubmed-meshheading:12020947-Deoxyribonucleases, Type II Site-Specific, pubmed-meshheading:12020947-Gene Expression, pubmed-meshheading:12020947-Humans, pubmed-meshheading:12020947-Infant, Newborn, pubmed-meshheading:12020947-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:12020947-Promoter Regions, Genetic, pubmed-meshheading:12020947-RNA, Messenger, pubmed-meshheading:12020947-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12020947-T-Lymphocytes, pubmed-meshheading:12020947-Transfection
pubmed:year
2002
pubmed:articleTitle
Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter.
pubmed:affiliation
Department of Medicine, Veterans Affairs Hospital, 5310 Cancer Center and Geriatrics Center Building, University of Michigan, Ann Arbor, MI 48109-0940, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.