Linked Life Data

12020944

Source:http://linkedlifedata.com/resource/pubmed/id/12020944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-5-21
pubmed:abstractText
The current studies were initiated to examine the expression and regulation of an oxidative stress-responsive transcription factor, NF-kappa B, in rat adrenals during aging. NF- kappa B DNA-binding activity and expression of constituent proteins (Rel family of proteins and I kappa Bs) was measured in adrenal nuclear and cytoplasmic extracts from young mature (5 month) and old (24 month) Sprague-Dawley rats before and after treatment with LPS; the latter was used to further invoke oxidative stress. Administration of LPS to either young or old rats induced a dramatic activation of NF- kappa B DNA binding activity as assayed by EMSA. NF- kappa B hetero-dimers, RelA/NF- kappa B1 (p65/p50) accounted for the majority of proteins that bound to consensus NF- kappa B sequences in LPS-treated young and old animals. The intensity of DNA binding complexes was significantly reduced in old animals. The age-related decline in the activation of NF- kappa B could not be attributed to an alteration in the composition of constituent subunits or degradation of NF- kappa B inhibitory proteins (I kappa B alpha and I kappa B beta) but rather was due to selective down-regulation of RelA/p65 and NF- kappa B2/p52 proteins. No age-related or LPS-induced changes in the constitutively active transcription factors SP-1 and OCT-1 were detected. These data suggest that aberrancies in the activation of NF- kappa B DNA-binding activity may contribute to the excessive oxidative damage observed in adrenal tissue with aging and may adversely affect cellular processes crucial for intracellular cholesterol transport and steroid hormone production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0047-6374
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1211-27
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Age-dependent modulation of NF-kappaB expression in rat adrenal gland.
pubmed:affiliation
Geriatric Research, Education and Clinical Center (GRECC, 182B), VA Palo Alto Health Care System, 3801 Miranda Ave, Palo Alto, CA 94304, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.