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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-5-20
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pubmed:abstractText |
This study examined polymorphisms in the dihydrofolate reductase (DHFR) gene of Pneumocystis carinii isolates from 27 patients with P. carinii pneumonia (PCP) in Japan. Four substitution sites with two synonymous and two non-synonymous changes were found. Two synonymous substitutions at nucleotide positions 540 and 312 were identified in one and 13 patients, respectively. Two amino acid substitutions (Ala67Val, Cysl66Tyr) were found in two different patients. No linkage of amino acid substitutions in DHFR to those in dihydropteroate synthase was observed. The two patients whose isolates showed non-synonymous DHFR mutations were not exposed to DHFR inhibitors before they developed PCP and were treated successfully with co-trimoxazole.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-2615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
510-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12018659-Amino Acid Sequence,
pubmed-meshheading:12018659-Anti-Infective Agents,
pubmed-meshheading:12018659-Base Sequence,
pubmed-meshheading:12018659-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:12018659-Dihydropteroate Synthase,
pubmed-meshheading:12018659-Gene Amplification,
pubmed-meshheading:12018659-Genes, Fungal,
pubmed-meshheading:12018659-Humans,
pubmed-meshheading:12018659-Immunocompromised Host,
pubmed-meshheading:12018659-Japan,
pubmed-meshheading:12018659-Molecular Sequence Data,
pubmed-meshheading:12018659-Mutation,
pubmed-meshheading:12018659-Pneumocystis,
pubmed-meshheading:12018659-Pneumonia, Pneumocystis,
pubmed-meshheading:12018659-Polymerase Chain Reaction,
pubmed-meshheading:12018659-Polymorphism, Genetic,
pubmed-meshheading:12018659-Sequence Alignment,
pubmed-meshheading:12018659-Sequence Analysis,
pubmed-meshheading:12018659-Tetrahydrofolate Dehydrogenase,
pubmed-meshheading:12018659-Treatment Failure,
pubmed-meshheading:12018659-Trimethoprim-Sulfamethoxazole Combination
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pubmed:year |
2002
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pubmed:articleTitle |
Dihydrofolate reductase gene polymorphisms in Pneumocystis carinii f. sp. hominis in Japan.
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pubmed:affiliation |
Division of Infectious Diseases, Advanced Clinical Research Center, University of Tokyo, Japan. takahata@ims.u-tokyo.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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