Linked Life Data

12016893

Source:http://linkedlifedata.com/resource/pubmed/id/12016893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-5-17
pubmed:abstractText
The relation between Ca2+/CaM PK II activity and excitatoxicity was studied in an in vitro model of rat hippocampal slices. The slices were exposed to 50-200 mumol.L-1 glutamate or 25-100 mumol.L-1 NMDA and glucose-free Krebs buffer for 30 min after being recovered to normal conditions by 2 hours of incubation with standard Krebs buffer. The results showed that inhibition of Ca2+/CaM PK II activity in rat hippocampal slices was induced by exogenous EAA (glutamate or NMDA), and Ca2+/CaM PK II activity values decreased to 50.1% and 44.7% of control at 200 mumol.L-1 glutamate and 100 mumol.L-1 NMDA, respectively; MK801, but not DNQX, antagonized EAA-induced inhibition of Ca2+/CaM PK II activity. When the slices were pretreated with MK801 prior to exposure to 200 mumol.L-1 glutamate or 100 mumol.L-1 NMDA, the Ca2+/Cam PK II activity values were 91.5% and 96.7%, respectively. The changes of extracellular Ca2+ or Mg2+ concentration influenced Ca2+/CaM PK II activity of the slices exposed to exogenous glutamate; Ca2+/CaM PK II activity values in the presence of extracellular Ca2+ was lower than that in the absence of extracellular Ca2+, and it changed from 50.1% of control (presence of Ca2+) to 64% of control (absence of Ca2+) at 200 mumol.L-1 glutamate, but Ca2+/CaM PK II activity values in the presence of extracellular Mg2+ was higher than that in the absence of extracellular Mg2+, and it changed from 50.1% of control(presence of Mg2+) to 36.6% of control (absence of Mg2+) at 200 mumol.L-1 glutamate. The results suggest that NMDA receptor may be involved in excitotoxicity-induced inhibition of Ca2+/CaM PK II activity.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0513-4870
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
561-5
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
[Effect of excitatoxicity on Ca2+/CaM PK II activity in rat hippocampal slices].
pubmed:affiliation
Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002.
pubmed:publicationType
Journal Article, In Vitro, English Abstract, Research Support, Non-U.S. Gov't