Source:http://linkedlifedata.com/resource/pubmed/id/12012026
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-5-15
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pubmed:abstractText |
The compound 2-aminoethoxydiphenyl borate (2-APB) has been used as either a specific membrane-permeable inhibitor for InsP(3) receptors or a store-operated Ca(2+) channel blocker in some cells. In this study, we have investigated actions of 2-APB on Ca(2+) signalling in mouse pancreatic acinar cells by measuring Ca(2+) concentration in the cytosol ([Ca(2+)]c) and in the endoplasmic reticulum (ER). Although 2-APB (50 microM) inhibited or modulated [Ca(2+)]c oscillations generated by a low dose of ACh, it did not block InsP(3)-mediated Ca(2+) release from the ER elicited by high doses of acetylcholine (ACh). 2-APB alone more than 70 microM tended to increase [Ca(2+)]c. When we directly measured Ca(2+) concentration in the lumen of the ER with a low affinity Ca(2+) dye, Mag-fluo-4, 2-APB itself slowly lowered ER Ca(2+) concentration and ACh could further release Ca(2+) from the ER in the presence of 2-APB, suggesting its lack of potency to block InsP(3) receptors. When store-operated Ca(2+) entry was evoked by addition of external Ca(2+) (5 mM) after depletion of Ca(2+) stores, 2-APB (50 microM) substantially blocked the Ca(2+) influx in a reversible manner. We conclude that (a) 2-APB is a good blocker for store-operated Ca(2+) channels, (b) 2-APB could not effectively block InsP(3) receptors, and (c) low doses of 2-APB lower Ca(2+) concentration in the lumen of the ER without a significant elevation of [Ca(2+)]c.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-aminoethoxydiphenyl borate,
http://linkedlifedata.com/resource/pubmed/chemical/Boron Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0028-1298
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
365
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-405
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pubmed:dateRevised |
2007-7-18
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pubmed:meshHeading |
pubmed-meshheading:12012026-Animals,
pubmed-meshheading:12012026-Boron Compounds,
pubmed-meshheading:12012026-Calcium,
pubmed-meshheading:12012026-Calcium Channel Blockers,
pubmed-meshheading:12012026-Calcium Channels,
pubmed-meshheading:12012026-Calcium Signaling,
pubmed-meshheading:12012026-Cells, Cultured,
pubmed-meshheading:12012026-Dose-Response Relationship, Drug,
pubmed-meshheading:12012026-Endoplasmic Reticulum,
pubmed-meshheading:12012026-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:12012026-Mice,
pubmed-meshheading:12012026-Pancreas,
pubmed-meshheading:12012026-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:12012026-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Slow depletion of endoplasmic reticulum Ca(2+) stores and block of store-operated Ca(2+) channels by 2-aminoethoxydiphenyl borate in mouse pancreatic acinar cells.
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pubmed:affiliation |
Department of Physiology, Sungkyunkwan University School of Medicine, 300 Chunchundong, Changanku, Suwon 440-746, Korea. mkpark@med.skku.ac.kr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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