Source:http://linkedlifedata.com/resource/pubmed/id/12010495
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2002-5-15
|
| pubmed:abstractText |
In a system described by Cairns and Foster, starvation of a particular leaky lac mutant (lacIZ33) in the presence of lactose appears to direct mutation in non-growing cells to sites that allow growth (adaptive mutation). This behaviour requires that the lac operon be located on an F' plasmid. This position effect was investigated by placing the mutant lac operon at many sites in the genome of Salmonella enterica (Typhimurium; LT2) and testing reversion behaviour. Genomic position did not affect reversion during non-selective growth. When lac was at any of 550 chromosomal sites, starvation caused little or no enhancement of reversion. In the 28 strains with the lac on Salmonella's conjugative plasmid (pSLT), selection enhanced reversion strongly, just as seen for strains with lac on an F' plasmid. In 46 strains, the lac operon was inserted within a small chromosomal duplication, and selection stimulated RecA-dependent partial reversion by simple amplification (about 8x) of the mutant lac region. The position of lac on a conjugative plasmid is important to reversion because it allows more frequent gene duplication and amplification. These events are central to growth and reversion under selection because they increase the number of replicating lac alleles within each developing revertant clone.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0950-382X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1017-32
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:12010495-Adaptation, Biological,
pubmed-meshheading:12010495-Chromosomes, Bacterial,
pubmed-meshheading:12010495-DNA Replication,
pubmed-meshheading:12010495-Escherichia coli,
pubmed-meshheading:12010495-Frameshift Mutation,
pubmed-meshheading:12010495-Gene Amplification,
pubmed-meshheading:12010495-Gene Duplication,
pubmed-meshheading:12010495-Gene Order,
pubmed-meshheading:12010495-Genes, Lethal,
pubmed-meshheading:12010495-Genome, Bacterial,
pubmed-meshheading:12010495-Lac Operon,
pubmed-meshheading:12010495-Lactose,
pubmed-meshheading:12010495-Mutagenesis,
pubmed-meshheading:12010495-Plasmids,
pubmed-meshheading:12010495-Rec A Recombinases,
pubmed-meshheading:12010495-Recombination, Genetic,
pubmed-meshheading:12010495-Salmonella enterica,
pubmed-meshheading:12010495-Selection, Genetic,
pubmed-meshheading:12010495-Suppression, Genetic
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
The effect of genomic position on reversion of a lac frameshift mutation (lacIZ33) during non-lethal selection (adaptive mutation).
|
| pubmed:affiliation |
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|