Linked Life Data

12009212

Source:http://linkedlifedata.com/resource/pubmed/id/12009212

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-5-15
pubmed:abstractText
Interferon gamma (IFN-gamma) has various immunoregulatory effects and its measurement is widely used for monitoring immune response in many clinical trials for cancer vaccines. In this study, we developed a fluorescent-linked immunosorbent assay (FLISA) for the measurement of IFN-gamma. The FLISA can accurately measure IFN-gamma concentrations and the linear dynamic range of standard curve is 15.6-2000 pg/ml (2.1 log range). Because the FLISA needs only a small amount of antibodies and there is no washing process, it is able to reduce hands-on time and running costs compared with traditional enzyme-linked immunosorbent assay (ELISA). The FLISA can be readily performed in both 96- and 384-well plates, and up to 60 plates can be handled automatically by a robotic arm. Measurement of IFN-gamma by fluorometric microvolume assay technology (FMAT) may be a high-throughput screening method for a large number of samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-76
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Measurement of interferon-gamma by high-throughput fluorometric microvolume assay technology system.
pubmed:affiliation
Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Japan. kom@med.kurume-u.ac.jp
pubmed:publicationType
Journal Article