Linked Life Data

12007862

Source:http://linkedlifedata.com/resource/pubmed/id/12007862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-5-14
pubmed:abstractText
A new screening method was developed for the detection of CAG expanded alleles in patients with hereditary ataxia using polymerase chain reaction-based microtiter plate hybridization (PCR-MPH). The system can be applied to detect pathologic alleles by hybridization with the immobilized (CAG)48 repeat probe derived from the unrelated gene 'ERDA1' except for the CAG repeats. We examined 10 individuals with SCA3, 10 with Huntington disease and 30 normal controls (31 controls for SCA3) using this method. The results showed that a clear discrimination was possible in all cases. We suggest that this system be made available for mass screening of patients with hereditary ataxia disorders. This report is the first to demonstrate that a PCR-MPH system can be successfully applied to DNA size differentiation in addition to base pair mismatches. Also, our design of the probe is unique in that the probe motif stem from the unrelated gene sequence and not from the synthetic oligonucleotides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-23
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A simple method for the detection of neurologic disorders associated with CAG repeat expansion using PCR-microtiter plate hybridization.
pubmed:affiliation
Department of Clinical Research Center, Samsung Biomedical Research Institute, Sungkyunkwan University, Samsung Medical Center, 50 Ilwon-dong, kangnam-ku, 135-710, Seoul, South Korea.
pubmed:publicationType
Journal Article, Clinical Trial, Evaluation Studies