|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2002-5-31
|
|
pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461364,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461365,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461368,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461369,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461370,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461371,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461372,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461373,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461374,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461375,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461376,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461377,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461378,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461379,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461380,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461381,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461382,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461383,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461386,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF461387,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M15825,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M80343,
http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_021605
|
|
pubmed:abstractText |
Long interspersed elements (LINE-1s) are abundant retrotransposons in mammalian genomes that probably retrotranspose by target site-primed reverse transcription (TPRT). During TPRT, the LINE-1 endonuclease cleaves genomic DNA, freeing a 3' hydroxyl that serves as a primer for reverse transcription of LINE-1 RNA by LINE-1 reverse transcriptase. The nascent LINE-1 cDNA joins to genomic DNA, generating LINE-1 structural hallmarks such as frequent 5' truncations, a 3' poly(A)+ tail and variable-length target site duplications (TSDs). Here we describe a pathway for LINE-1 retrotransposition in Chinese hamster ovary (CHO) cells that acts independently of endonuclease but is dependent upon reverse transcriptase. We show that endonuclease-independent LINE-1 retrotransposition occurs at near-wildtype levels in two mutant cell lines that are deficient in nonhomologous end-joining (NHEJ). Analysis of the pre- and post-integration sites revealed that endonuclease-independent retrotransposition results in unusual structures because the LINE-1s integrate at atypical target sequences, are truncated predominantly at their 3' ends and lack TSDs. Moreover, two of nine endonuclease-independent retrotranspositions contained cDNA fragments at their 3' ends that are probably derived from the reverse transcription of endogenous mRNA. Thus, our results suggest that LINE-1s can integrate into DNA lesions, resulting in retrotransposon-mediated DNA repair in mammalian cells.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1061-4036
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
31
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
159-65
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
DNA repair mediated by endonuclease-independent LINE-1 retrotransposition.
|
|
pubmed:affiliation |
Department of Human Genetics, University of Michigan Medical School, 1241 E. Catherine Street, Ann Arbor, Michigan 48105-0618, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
