Linked Life Data

12006486

Source:http://linkedlifedata.com/resource/pubmed/id/12006486

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2002-5-13
pubmed:abstractText
Membrane-bound receptors such as tyrosine kinases and ionotropic receptors are associated with large protein networks structured by protein-protein interactions involving multidomain proteins. Although these networks have emerged as a general mechanism of cellular signalling, much less is known about the protein complexes associated with G-protein-coupled receptors (GPCRs). Using a proteomic approach based on peptide affinity chromatography followed by mass spectrometry and immunoblotting, we have identified 15 proteins that interact with the C- terminal tail of the 5-hydroxytryptamine 2C (5-HT(2C)) receptor, a GPCR. These proteins include several synaptic multidomain proteins containing one or several PDZ domains (PSD95 and the proteins of the tripartite complex Veli3-CASK-Mint1), proteins of the actin/spectrin cytoskeleton and signalling proteins. Coimmunoprecipitation experiments showed that 5-HT(2C) receptors interact with PSD95 and the Veli3-CASK-Mint1 complex in vivo. Electron microscopy also indicated a synaptic enrichment of Veli3 and 5-HT(2C) receptors and their colocalization in microvilli of choroidal cells. These results indicate that the 5-HT(2C) receptor is associated with protein networks that are important for its synaptic localization and its coupling to the signalling machinery.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10341223, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10352011, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10373412, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10414974, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10488080, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10551867, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10636891, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10646521, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10657302, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10665825, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10712924, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10753959, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-10862698, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11031113, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11036064, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11050113, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11104771, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11149929, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11150294, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11185985, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11217851, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11283303, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11311377, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11339973, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11343921, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11431030, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11583808, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11604393, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-11707406, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-1455506, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-2709329, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-3467321, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-7531308, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-7798914, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-8612272, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-8625413, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-8779443, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-8788961, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9395480, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9425014, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9537516, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9546224, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9560162, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9605579, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9660868, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9753323, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9753324, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9796815, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9822620, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9838122, http://linkedlifedata.com/resource/pubmed/commentcorrection/12006486-9952408
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2332-42
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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