Linked Life Data

12004977

Source:http://linkedlifedata.com/resource/pubmed/id/12004977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-5-13
pubmed:abstractText
The aim of the present study was to demonstrate induction of vitellogenin in the common carp (Cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens of common carp were treated experimentally with 17beta-estradiol and increasing doses of 4-nonylphenol and levels of plasma Vtg were measured in order to: 1) validate an ELISA assay for plasma Vtg in the common carp using the polyclonal rabbit anti-salmon Vtg antibody AA1 (Biosense, Norway); 2) check the sensitivity of carp juveniles in producing Vtg in response to estrogen stimulation. The group treated with 17beta-estradiol showed high induction (156%) with respect to controls, also groups treated with 4-nonylphenol showed induction of Vtg. The group treated with 100 mg kg(-1) b.wt showed an induction of 61%. A statistically significant correlation was found between dose and response. This preliminary study demonstrate a response to the ELISA assay for Vtg in the common carp using rabbit anti-salmon antibody AA1. With further evidence the tested biomarker might be proposed for large scale monitoring of estrogenic effects caused by pollution in urban and industrial effluents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0167-6369
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-9
pubmed:dateRevised
2009-5-11
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Vitellogenin induction in Cyprinus carpio treated with 17beta-estradiol and 4-nonylphenol.
pubmed:affiliation
Department of Environmental Science, University of Siena, Italy. CasiniS@unisi.it
pubmed:publicationType
Journal Article