Linked Life Data

12004059

Source:http://linkedlifedata.com/resource/pubmed/id/12004059

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
2002-7-22
pubmed:abstractText
The POZ domain is a protein-protein interaction motif that is found in many transcription factors, which are important for development, oncogenesis, apoptosis, and transcription repression. We cloned the POZ domain transcription factor, FBI-1, that recognizes the cis-element (bp -38 to -22) located just upstream of the core Sp1 binding sites (bp -22 to +22) of the ADH5/FDH minimal promoter (bp -38 to +61) in vitro and in vivo, as revealed by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. The ADH5/FDH minimal promoter is potently repressed by the FBI-1. Glutathione S-transferase fusion protein pull-down showed that the POZ domains of FBI-1, Plzf, and Bcl-6 directly interact with the zinc finger DNA binding domain of Sp1. DNase I footprinting assays showed that the interaction prevents binding of Sp1 to the GC boxes of the ADH5/FDH promoter. Gal4-POZ domain fusions targeted proximal to the GC boxes repress transcription of the Gal4 upstream activator sequence-Sp1-adenovirus major late promoter. Our data suggest that POZ domain represses transcription by interacting with Sp1 zinc fingers and by interfering with the DNA binding activity of Sp1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26761-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12004059-Aldehyde Oxidoreductases, pubmed-meshheading:12004059-Amino Acid Sequence, pubmed-meshheading:12004059-Animals, pubmed-meshheading:12004059-Cell Nucleus, pubmed-meshheading:12004059-Chromatin, pubmed-meshheading:12004059-DNA, pubmed-meshheading:12004059-DNA-Binding Proteins, pubmed-meshheading:12004059-Deoxyribonuclease I, pubmed-meshheading:12004059-Gene Library, pubmed-meshheading:12004059-HeLa Cells, pubmed-meshheading:12004059-Humans, pubmed-meshheading:12004059-Mice, pubmed-meshheading:12004059-Molecular Sequence Data, pubmed-meshheading:12004059-Multigene Family, pubmed-meshheading:12004059-Plasmids, pubmed-meshheading:12004059-Polymerase Chain Reaction, pubmed-meshheading:12004059-Precipitin Tests, pubmed-meshheading:12004059-Promoter Regions, Genetic, pubmed-meshheading:12004059-Protein Binding, pubmed-meshheading:12004059-Protein Structure, Tertiary, pubmed-meshheading:12004059-Sequence Homology, Amino Acid, pubmed-meshheading:12004059-Sp1 Transcription Factor, pubmed-meshheading:12004059-Transcription, Genetic, pubmed-meshheading:12004059-Transcription Factors, pubmed-meshheading:12004059-Zinc Fingers
pubmed:year
2002
pubmed:articleTitle
POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, BK21 Project for Medical Sciences, Institute of Genetic Sciences, Yonsei University School of Medicine, 134 ShinChon-Dong, SeoDaeMoon-Ku, Seoul 120-752, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't