|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2002-5-10
|
|
pubmed:abstractText |
The present study was designed to investigate the interaction between 5-methyltetrahydrofolate and tetrahydrobiopterin in modulating endothelial function. Tetrahydrobiopterin is a critical cofactor for nitric oxide synthase and maintains this enzyme as a nitric oxide- versus superoxide-producing enzyme. The structure of 5-methyltetrahydrofolate is similar to tetrahydrobiopterin and both agents have been shown to improve endothelium-dependent vasodilatation. We hypothesized that 5-methyltetrahydrofolate interacts with nitric oxide synthase in a fashion analogous, yet independent, of tetrahydrobiopterin to improve endothelial function. We demonstrate that 5-methyltetrahydrofolate binds the active site of nitric oxide synthase and mimics the orientation of tetrahydrobiopterin. Furthermore, 5-methyltetrahydrofolate attenuates superoxide production (induced by inhibition of tetrahydrobiopterin synthesis) and improves endothelial function in aortae isolated from tetrahydrobiopterin-deficient rats. We suggest that 5-methyltetrahydrofolate directly interacts with nitric oxide synthase to promote nitric oxide (vs. superoxide) production and improve endothelial function. 5-Methyltetrahydrofolate may represent an important strategy for intervention aimed at improving tetrahydrobiopterin bioavailability.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5,6,7,8-tetrahydrobiopterin,
http://linkedlifedata.com/resource/pubmed/chemical/5-methyltetrahydrofolate,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Biopterin,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III,
http://linkedlifedata.com/resource/pubmed/chemical/Pteridines,
http://linkedlifedata.com/resource/pubmed/chemical/Pterins,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamines,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrahydrofolates,
http://linkedlifedata.com/resource/pubmed/chemical/dihydrorhodamine 123,
http://linkedlifedata.com/resource/pubmed/chemical/sepiapterin
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0363-6135
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
282
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
H2167-72
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12003825-Acetylcholine,
pubmed-meshheading:12003825-Animals,
pubmed-meshheading:12003825-Aorta,
pubmed-meshheading:12003825-Binding Sites,
pubmed-meshheading:12003825-Biopterin,
pubmed-meshheading:12003825-Cattle,
pubmed-meshheading:12003825-Endothelium, Vascular,
pubmed-meshheading:12003825-Fluorescent Dyes,
pubmed-meshheading:12003825-Free Radicals,
pubmed-meshheading:12003825-Immunohistochemistry,
pubmed-meshheading:12003825-Nitric Oxide,
pubmed-meshheading:12003825-Nitric Oxide Synthase,
pubmed-meshheading:12003825-Nitric Oxide Synthase Type III,
pubmed-meshheading:12003825-Pteridines,
pubmed-meshheading:12003825-Pterins,
pubmed-meshheading:12003825-Reactive Oxygen Species,
pubmed-meshheading:12003825-Rhodamines,
pubmed-meshheading:12003825-Superoxides,
pubmed-meshheading:12003825-Tetrahydrofolates,
pubmed-meshheading:12003825-Vasodilation
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Interaction of 5-methyltetrahydrofolate and tetrahydrobiopterin on endothelial function.
|
|
pubmed:affiliation |
Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
