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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-5-10
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pubmed:abstractText |
Histamine increases microvascular permeability by creating small transitory (100-400 nm) gaps between adjacent endothelial cells at sites of vascular endothelial (VE)-cadherin-based adhesion. We examined the effects of histamine on the proteins within the VE-cadherin-based adherens junction in primary human umbilical vein endothelial cells. VE-cadherin is linked not only by beta- and alpha-catenin to cortical actin but also by gamma-catenin to the intermediate filament vimentin. In mature human umbilical vein cultures, the VE-cadherin immunoprecipitate contained equivalent amounts of alpha- and beta-catenin, 130% as much beta- as gamma-catenin, and 50% as much actin as vimentin. Within 60 s, histamine decreased the fraction of VE-cadherin in the insoluble portion of the cell lysate by 35 +/- 1.5%. At the same time, histamine decreased the amount of vimentin that immunoprecipitated with VE-cadherin by 50 +/- 6%. Histamine did not affect the amount of actin or the amount of alpha-, beta-, or gamma-catenin that immunoprecipitated with VE-cadherin. Within 60 s, histamine simulated a doubling in the phosphorylation of VE-cadherin and beta- and gamma-catenin. The VE-cadherin immunoprecipitate contained kinase activity that phosphorylated VE-cadherin and gamma-catenin in vitro.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Desmoplakins,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/JUP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Vimentin,
http://linkedlifedata.com/resource/pubmed/chemical/alpha Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/cadherin 5,
http://linkedlifedata.com/resource/pubmed/chemical/gamma Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1040-0605
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L1330-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12003790-Actins,
pubmed-meshheading:12003790-Adherens Junctions,
pubmed-meshheading:12003790-Antigens, CD,
pubmed-meshheading:12003790-Cadherins,
pubmed-meshheading:12003790-Cells, Cultured,
pubmed-meshheading:12003790-Cytoskeletal Proteins,
pubmed-meshheading:12003790-Cytoskeleton,
pubmed-meshheading:12003790-Desmoplakins,
pubmed-meshheading:12003790-Endothelium, Vascular,
pubmed-meshheading:12003790-Histamine,
pubmed-meshheading:12003790-Humans,
pubmed-meshheading:12003790-Membrane Proteins,
pubmed-meshheading:12003790-Phosphorylation,
pubmed-meshheading:12003790-Precipitin Tests,
pubmed-meshheading:12003790-Trans-Activators,
pubmed-meshheading:12003790-Vimentin,
pubmed-meshheading:12003790-alpha Catenin,
pubmed-meshheading:12003790-beta Catenin,
pubmed-meshheading:12003790-gamma Catenin
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pubmed:year |
2002
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pubmed:articleTitle |
Histamine stimulates phosphorylation of adherens junction proteins and alters their link to vimentin.
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pubmed:affiliation |
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA. michael.shasby@uiowa.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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