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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-5-9
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pubmed:abstractText |
The effects of purified toxin A in vitro on the shape and function of polymorphonuclear leukocytes (PMNL) were examined. Toxin A induced changes in adherent PMNL shape from a compact spherical or pyramidal shape to a thin and rope-like shape. This change in shape was accompanied by rearrangement of the F-actin cytoskeleton into aggregates. Toxin A-treated PMNL exhibited increased adherence and expressed less L-selectin and more Mac-1, compared with untreated PMNL. In contrast to these proinflammatory actions, toxin A impaired both directed and non-directed PMNL migration in response to N-formylmethionylleucylphenylalanine. In addition, toxin A decreased the oxidative activity of adherent PMNL stimulated by recombinant human tumor necrosis factor-alpha. These effects could be explained by toxin A-induced glucosylation of the signaling small-size guanine 5'-triphosphate-binding proteins of the Rho family in human PMNL.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1297-306
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12001047-Bacterial Toxins,
pubmed-meshheading:12001047-Cell Adhesion,
pubmed-meshheading:12001047-Cell Movement,
pubmed-meshheading:12001047-Chemotaxis, Leukocyte,
pubmed-meshheading:12001047-Cytochalasin B,
pubmed-meshheading:12001047-Cytoskeleton,
pubmed-meshheading:12001047-Dose-Response Relationship, Drug,
pubmed-meshheading:12001047-Enterotoxins,
pubmed-meshheading:12001047-Glycosylation,
pubmed-meshheading:12001047-Humans,
pubmed-meshheading:12001047-Macrophage-1 Antigen,
pubmed-meshheading:12001047-Microscopy, Electron, Scanning,
pubmed-meshheading:12001047-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:12001047-Neutrophils,
pubmed-meshheading:12001047-Tumor Necrosis Factor-alpha,
pubmed-meshheading:12001047-rho GTP-Binding Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Clostridium difficile toxin A alters in vitro-adherent neutrophil morphology and function.
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pubmed:affiliation |
Department of Morphology, Federal University of Ceará, Fortaleza, Brazil
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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